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Phospho-AR/Androgen receptor (

Ser650) Rabbit Polyclonal Antibody抗体，orb4542，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb4542
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      Phospho-AR/Androgen receptor (Ser650) Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      Phospho-AR/Androgen receptor (Ser650) Rabbit Polyclonal Antibody

    • 靶点：

      AR

    • 浓度：

      1mg/ml

    • 应用范围：

      FC, IF, IHC-Fr, IHC-P

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb4542

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated Synthesised phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser650 TT(p-S)PT

    • 规格：

      50 ul

    产品描述：Phospho-AR/Androgen receptor (Ser650) Rabbit Polyclonal Antibody

    别名:Androgen Receptor(phospho S650); Androgen Receptor(phospho-Ser650); Androgen Receptor(phospho S650); p-Androgen Receptor(Ser650); ANDR_HUMAN; HYSP1; AIS; Androgen receptor(dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease); AR; DHTR; Dihydro Testosterone Receptor; Dihydrotestosterone receptor; HUMARA; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; Spinal and bulbar muscular atrophy; TFM.

    免疫原：KLH conjugated Synthesised phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser650 TT(p-S)PT

    预测反应性:Canine, Porcine, Rabbit

    分子量:43/101 kDa

    应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P10275

    Note:For research use only.

    产品细节图片1
    Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-phospho-Androgen Receptor (Ser650) antibody (orb4542), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Androgen Receptor (Ser650)) Polyclonal Antibody, Unconjugated (orb4542) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ANDR) Polyclonal Antibody, Unconjugated (orb4542) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ANDR) Polyclonal Antibody, Unconjugated (orb4542) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片5
    Fixative-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ANDR) Polyclonal Antibody, Unconjugated (orb4542) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片6
    Fixative-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Androgen Receptor (Ser650)) Polyclonal Antibody, Unconjugated (orb4542) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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