- ￥3120
- biorbyt
- orb5127
- 英国
- 2025年12月04日
- ICC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
Phospho-EGFR (Tyr1110) Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Phospho-EGFR (Tyr1110) Rabbit Polyclonal Antibody
- 靶点：
EGFR
- 浓度：
1mg/ml
- 应用范围：
ICC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb5127
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Tyr1110 PV(p-Y)HN
- 规格：
50 ul
产品描述：Phospho-EGFR (Tyr1110) Rabbit Polyclonal Antibody
别名:Avian erythroblastic leukemia viral(v erb b) oncogene homolog; Avian erythroblastic leukemia viral(verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; Epidermal growth factor receptor(avian erythroblastic leukemia viral(v erb b) oncogene homolog); Epidermal growth factor receptor(erythroblastic leukemia viral(v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Tyr1110 PV(p-Y)HN
预测反应性:Bovine, Equine, Porcine, Rabbit
分子量:134 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P00533
Note:For research use only.
别名:Avian erythroblastic leukemia viral(v erb b) oncogene homolog; Avian erythroblastic leukemia viral(verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; Epidermal growth factor receptor(avian erythroblastic leukemia viral(v erb b) oncogene homolog); Epidermal growth factor receptor(erythroblastic leukemia viral(v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Tyr1110 PV(p-Y)HN
预测反应性:Bovine, Equine, Porcine, Rabbit
分子量:134 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P00533
Note:For research use only.
Hela cell, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-EGFR (Tyr1110)) polyclonal Antibody, Unconjugated (orb5127) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Fixative-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-EGFR (Tyr1110)) Polyclonal Antibody, Unconjugated (orb5127) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (Mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-EGFR (Tyr1110)) Polyclonal Antibody, Unconjugated (orb5127) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Rat serum at 40 ug, Rat plasma at 40 ug, Primary: Anti-phospho-EGFR (Tyr1110) (orb5127) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 134 kD, Observed band size: 134 kD.
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Phospho-EGFR (Tyr1110) Rabbit Polyclonal Antibody抗体，orb5127，biorbyt
￥3120
