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NET1 Rabbit Polyclonal Antibod

y抗体,orb157950,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb157950
  • 英国
  • 2025年12月04日
  • IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      NET1 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      NET1 Rabbit Polyclonal Antibody

    • 靶点

      NET1

    • 浓度

      1mg/ml

    • 应用范围

      IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb157950

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human NET1 (151-250/596aa)

    • 规格

      50 ul

    产品描述:NET1 Rabbit Polyclonal Antibody

    别名:Neuroepithelial cell-transforming gene 1 protein; Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8; ARHGEF8; ARHG8_HUMAN;

    免疫原:KLH conjugated synthetic peptide derived from human NET1 (151-250/596aa)

    预测反应性:Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep

    分子量:65 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q7Z628

    Note:For research use only.

    产品细节图片1
    HepG2 cell, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (NET1) polyclonal Antibody, Unconjugated (orb157950) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片2
    Sample: Jurkat (Human) Cell Lysate at 30 ug, Skin (Mouse) Lysate at 40 ug, Skin (Rat) Lysate at 40 ug, Primary: Anti-NET1 (orb157950) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.

    产品细节图片3
    Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Mouse Heart tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Cerebellum tissue lysates, Lane 6: Rat Stomach tissue lysates, Lane 7: Human K562 cell lysates, Lane 8: Human A431 cell lysates, Lane 9: Human HepG2 cell lysates, Lane 10: Human MCF-7 cell lysates, Primary: Anti-NET1 (orb157950) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kDa, Observed band size: 62 kDa.

    产品细节图片4
    Tissue/Cell: mouse brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NET1 Polyclonal Antibody, Unconjugated (orb157950) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
    相关实验
    • Polyclonal Antibody Production

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    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Preparation of Polyclonal Antibodies to Retinoid Receptors

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