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Alpha-synuclein (nitro-Tyr39)

Rabbit Polyclonal Antibody抗体,orb155648,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb155648
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Alpha-synuclein (nitro-Tyr39) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Alpha-synuclein (nitro-Tyr39) Rabbit Polyclonal Antibody

    • 靶点

      SNCA

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb155648

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised nitrylpeptide derived from human SNCA around the nitrosation site of Tyr39 VL(nitrated-Y)VG

    • 规格

      50 ul

    产品描述:Alpha-synuclein (nitro-Tyr39) Rabbit Polyclonal Antibody

    别名:SYUA_HUMAN; nitrated alpha-synuclein(nitrated-Tyr39); alpha-synuclein 3-nitrotyrosines(Tyr39); alpha-synuclein 3-nitrotyrosines Tyr39; alpha synuclein 3-nitrotyrosines Tyr39; nitrated alpha-synuclein(nitro-Tyr39); alpha-synuclein(nitro-Tyr39); alpha-synuclein(nitro Tyr39); SNCA; SYUA_HUMAN; alpha synuclein; alpha-synuclein, isoform NACP140; alpha SYN; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid precursor; Non-A-beta component of alzheimers disease amyloid, precursor of; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease(autosomal dominant, Lewy body) 4; Parkinson disease familial 1; Syn; Snca synuclein, alpha(non A4 component of amyloid precursor); Synuclein alpha; Synuclein, alpha(non A4 component of amyloid precursor); Synuclein-α; Synuclein α.

    免疫原:KLH conjugated Synthesised nitrylpeptide derived from human SNCA around the nitrosation site of Tyr39 VL(nitrated-Y)VG

    预测反应性:Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep

    分子量:15 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P37840

    Note:For research use only.

    产品细节图片1
    Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-Alpha-synuclein (nitro-Tyr39) antibody (orb155648), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Alpha-synuclein (nitro-Tyr39)) Polyclonal Antibody, Unconjugated (orb155648) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Alpha-synuclein (nitro-Tyr39)) Polyclonal Antibody, Unconjugated (orb155648) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (rat kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Alpha-synuclein (nitro-Tyr39)) Polyclonal Antibody, Unconjugated (orb155648) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

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    图标文献和实验
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      that was an interspecific hybrid (H) between Mus musculus domesticus (D, paternal genome) and Mus spretus (S, maternal genome). The native chromatin was then immunoprecipitated with rabbit polyclonal antisera to acetylation at lysine 9 of H3 (H3K9Ac) and to dimethylation

    • Antigen Design Sera Purification Tech Sheet

      should not be included in the sequence selected for antibody generation. Algorithms for predicting protein characteristics such as hydrophilicity/hydrophobicity and secondary structure regions such as alpha-helix, beta-sheet and beta-turn aid selection

    • Antigen Design Sera Purification Tech Sheet

      for antibody generation. Algorithms for predicting protein characteristics such as hydrophilicity/hydrophobicity and secondary structure regions such as alpha-helix, beta-sheet and beta-turn aid selection of a potentially exposed, immunogenic internal

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