- ￥8450
- biorbyt
- orb150849
- 英国
- 2025年12月04日
- ICC, IF, IHC, WB
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
LGI1 Antibody (Biotin)抗体
- 抗体英文名：
LGI1 Antibody (Biotin)
- 靶点：
LGI1
- 浓度：
1 mg/ml
- 应用范围：
ICC, IF, IHC, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb150849
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Biotin
- 克隆性：
Monoclonal
- 亚型：
IgG2a
- 免疫原：
Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.
- 规格：
100 ug
别名:LGI1, Leucine-rich glioma-inactivated protein 1, Epitempin 1, EPT, ETL1, ADLTE, ADPEAF, ADPAEF, IB1099, OTTHUMP00000020121, OTTHUMP00000020122
免疫原：Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.
克隆性:N283/7 (Formerly sold as S283-7)
分子量:60kDa
应用稀释比例：WB (1:1000)
应用注释：1 µg/ml of SMC-461 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
防腐剂:136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
纯化:Protein G Purified
保存说明:Conjugated antibodies should be stored according to the product label
NCBI:NP_064674.1
Entrez:56839
UniProt ID:Q9JIA1
Note:For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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