NR2A Antibody (Biotin) 抗体,orb150405,Biorbyt产品图

NR2A Antibody (Biotin) 抗体,orb1

50405,Biorbyt
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  • ¥7605
  • Biorbyt已认证
  • orb150405
  • 英国
  • 2026年07月28日
  • ICC, IF, IHC, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      NR2A Antibody (Biotin) 抗体

    • 抗体英文名:

      NR2A Antibody (Biotin)

    • 靶点:

      NR2A

    • 浓度:

      1 mg/ml

    • 应用范围:

      ICC, IF, IHC, WB

    • 宿主:

      Mouse

    • 适应物种:

      Human, Mouse, Rat

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb150405

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Biotin

    • 克隆性:

      Monoclonal

    • 保存条件:

      详询

    • 亚型:

      IgG2b

    • 免疫原:

      详询

    • 规格:

      100 ug

    类别: Antibodies

    描述: Mouse monoclonal antibody against GRIN2A conjugated to Biotin

    克隆号: N327A/38

    别名: NMDA 2A, NMDAR2A, NMDAR 2A, NMDA Receptor 2A, Glutamate Receptor, GRIN2A, Glutamate [NMDA] Receptor subunit epsilon-1, Glutamate receptor ionotropic N methyl D aspartate 2A, HNR2A, N methyl D aspartate receptor channel, subunit epsilon 1, N Methyl D Aspartate Receptor Subtype 2A, N methyl D aspartate receptor subunit 2A, NMDA receptor subtype 2A, NMDA Receptor Type 2A, OTTHUMP00000160135, OTTHUMP00000174531

    应用稀释比例: WB (1:1000), ICC/IF (1:100)

    应用说明: 1 µg/ml was sufficient for detection of GluN2A in 20 µg of COS cells transiently transfected with GFP-tagged NR2A lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    免疫原: Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A

    分子量: 170kDa

    防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate

    纯化方法: Protein G Purified

    特异性: Detects ~170kDa. Does not react with NR2B.

    研究领域: Neuroscience

    UniProt ID: Q00959

    NCBI 参考序列: NP_036705.3

    Entrez 基因 ID: 24409

    保存条件: Conjugated antibodies should be stored according to the product label

    备注: For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone N327A/38. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NR2A Antibody (D) Composite.

    产品细节图片2
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone N327A/38. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-GluN2A/NR2A Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN2A/NR2A Antibody. (D) Composite.

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    图标文献和实验
    相关实验
    • The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods

      These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent

    • The Avidin-Biotin Complex(ABC) Method

      , between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin

    • Biotinylation of Antibody

      sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room

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