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ASIC1 Antibody (FITC)抗体,orb150

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  • ¥8450
  • biorbyt已认证
  • orb150287
  • 英国
  • 2025年12月04日
  • ICC, IF, IHC, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      ASIC1 Antibody (FITC)抗体

    • 抗体英文名

      ASIC1 Antibody (FITC)

    • 靶点

      ASIC1

    • 浓度

      1 mg/ml

    • 应用范围

      ICC, IF, IHC, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb150287

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      FITC

    • 克隆性

      Monoclonal

    • 亚型

      IgG1

    • 免疫原

      Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1

    • 规格

      100 ug

    产品描述:Mouse monoclonal to ASIC1 (FITC). Acid sensing ion channel ASIC1, also designated ACCN2, BNAC2 and ASIC1a, is present in brain as a 4. 3-kb transcript with localization to rat dorsal root ganglia. In situ hybridization of rat brain suggests that ASIC1 is most abundant in the main olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei and cerebellum. ASIC1 and H+-gated currents may contribute to the development of fear and anxiety..

    别名:ASIC1, ACCN2, BNAC2, Acid-sensing ion channel 1, Acid-sensing (proton-gated) ion channel, Amiloride-sensitive cation channel 2 neuronal, Brain sodium channel 2

    免疫原:Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1

    克隆性:N271/44 (Formerly sold as S271-44)

    分子量:60kDa

    应用稀释比例:WB (1:1000)

    应用注释:1 µg/ml of SMC-427 was sufficient for detection of ASIC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

    纯化:Protein G Purified

    保存说明:Conjugated antibodies should be stored according to the product label

    NCBI:NP_033727.1

    Entrez:11419

    UniProt ID:Q6NXK8

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ASIC1 Monoclonal Antibody, Clone N271/44. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ASIC1 Antibody (D) Composite.

    产品细节图片2
    Western Blot analysis of Rat brain lysates showing detection of ASIC1 protein using Mouse Anti-ASIC1 Monoclonal Antibody, Clone N271/44. Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody at 1:100, 1:250, 1:500, and 1:1000.

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    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • secondary antibody review -- data from 99 publications

      localization of tagged SAX-3 in 293T cell Molecular Probes 2       Alexa Fluor 647 immunocytochemistry   localization of BCG phagosomes   3       Alexa Fluor 647 immunohistochemistry   detect surface DFz2   3       FITC immunohistochemistry   determine

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