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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
SLC38A1 Antibody (HRP)抗体
- 抗体英文名:
SLC38A1 Antibody (HRP)
- 靶点:
SLC38A1
- 浓度:
1 mg/ml
- 应用范围:
FC, ICC, IF, IHC, WB
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb149846
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
HRP
- 克隆性:
Monoclonal
- 亚型:
IgG1
- 免疫原:
Fusion protein amino acids 1- 63 of rat SNAT1
- 规格:
100 ug
别名:SNAT1, SLC38A1, amino acids transporter A1, ATA1, NAT2, sodium coupled neutral amino acids transporter 1, Amino acid transporter system A1, N-system amino acid transporter 2, S38A1, SAT1, SNAT 1, Solute carrier family 38 member 1, System A amino acid transporter 1, System N amino acid transporter 1
免疫原:Fusion protein amino acids 1- 63 of rat SNAT1
克隆性:N104/32 (Formerly sold as S104-32)
分子量:50kDa
应用稀释比例:WB (1:1000)
应用注释:1 µg/ml of SMC-401 was sufficient for detection of SNAT1 in 20 µg of lysates from neocortical neurons cultured under amino acid starvation conditions and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
防腐剂:73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
纯化:Protein G Purified
保存说明:Conjugated antibodies should be stored according to the product label
NCBI:NP_620187.1
Entrez:170567
UniProt ID:Q9JM15
Note:For research use only.


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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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