产品封面图

MDC1 Antibody (FITC)抗体,orb1480

43,biorbyt
收藏
  • ¥8060
  • biorbyt已认证
  • orb148043
  • 英国
  • 2025年12月04日
  • ICC, IF, WB
  • Mouse
  • Bovine, Human, Mouse, Primate
avatar
品牌商
13钻石会员
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      MDC1 Antibody (FITC)抗体

    • 抗体英文名

      MDC1 Antibody (FITC)

    • 靶点

      MDC1

    • 浓度

      1 mg/ml

    • 应用范围

      ICC, IF, WB

    • 宿主

      Mouse

    • 适应物种

      Bovine, Human, Mouse, Primate

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb148043

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      FITC

    • 克隆性

      Monoclonal

    • 亚型

      IgG1

    • 免疫原

      GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus

    • 规格

      100 ug

    产品描述:Mouse monoclonal to MDC1 (FITC). MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A. X on S139..

    别名:MDC1, Nuclear factor with BRCT domains1, Mediator of DNA damage checkpoint 1

    免疫原:GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus

    克隆性:P2B11

    分子量:184kDa

    应用稀释比例:WB (1:2000), ICC/IF (1:100)

    应用注释:0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.

    防腐剂:640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

    纯化:Protein G Purified

    保存说明:Conjugated antibodies should be stored according to the product label

    NCBI:NP_001010833.2

    Entrez:240087

    UniProt ID:Q5PSV9

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

    产品细节图片2
    Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.

    产品细节图片3
    Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • secondary antibody review -- data from 99 publications

      localization of tagged SAX-3 in 293T cell Molecular Probes 2       Alexa Fluor 647 immunocytochemistry   localization of BCG phagosomes   3       Alexa Fluor 647 immunohistochemistry   detect surface DFz2   3       FITC immunohistochemistry   determine

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    ¥2352
    武汉博欧特生物科技有限公司
    2025年06月01日询价
    ¥4006.80
    深圳欣博盛生物科技有限公司
    2025年03月31日询价
    询价
    南京赛戈巍生物科技有限公司
    2026年03月16日询价
    询价
    StressMarq Biosciences Inc.
    2026年02月05日询价
    ¥3342.75
    西宝生物科技(上海)股份有限公司
    2025年07月07日询价
    MDC1 Antibody (FITC)抗体,orb148043,biorbyt
    ¥8060