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YB1 Rabbit Polyclonal Antibody

抗体,orb100303,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb100303
  • 英国
  • 2025年12月04日
  • IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      YB1 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      YB1 Rabbit Polyclonal Antibody

    • 靶点

      YBX1

    • 浓度

      1mg/ml

    • 应用范围

      IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb100303

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human YB1 (51-150/324aa)

    • 规格

      50 ul

    产品描述:YB1 Rabbit Polyclonal Antibody

    别名:BP 8; CBF-A; binding transcription factor I subunit A; YBX1; CCAAT-binding transcription factor I subunit A; CSDA2; CSDB; DBPB; DNA binding protein B; DNA-binding protein B; EFI-A; Enhancer factor I subunit A; MDR NF1; NSEP 1; NSEP1; Nuclease sensitive element binding protein 1; Nuclease-sensitive element-binding protein 1; p50; Q15905; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB 1. YB-1; YBOX1_HUMAN; YBX 1; YBX1.

    免疫原:KLH conjugated synthetic peptide derived from human YB1 (51-150/324aa)

    预测反应性:Bovine, Canine, Gallus, Porcine, Rabbit

    分子量:36 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P67809

    Note:For research use only.

    产品细节图片1
    paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (YB1) Polyclonal Antibody, Unconjugated (orb100303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片2
    Fixative-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (YB1) Polyclonal Antibody, Unconjugated (orb100303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human HL-60 cell lysates, Lane 5: Human U251 cell lysates, Primary: Anti-YB1 (orb100303) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kDa, Observed band size: 50 kDa.

    产品细节图片4
    Tissue/cell: human glioma tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-YBX-1/YB1 Polyclonal Antibody, Unconjugated (orb100303) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
    相关实验
    • Polyclonal Antibody Production

      Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed        

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Preparation of Polyclonal Antibodies to Retinoid Receptors

      rabbit-antibody production and affinity purification. Suggestions for potential applications in the study of retinoid-molecular biology are also discussed.

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