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- 详细信息
- 技术资料
- 抗体名:
HPGD Rabbit Polyclonal Antibody抗体
- 抗体英文名:
HPGD Rabbit Polyclonal Antibody
- 靶点:
HPGD
- 浓度:
1mg/ml
- 应用范围:
FC, IF, IHC-Fr, IHC-P, WB
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb100318
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human Prostaglandin dehydrogenase 1 (55-160/266aa)
- 规格:
50 ul
产品描述:HPGD Rabbit Polyclonal Antibody
别名:PGDH_HUMAN; 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; EC:1.1.1.141; 15-PGDH; 15 PGDH; 15PGDH; Eicosanoid/docosanoid dehydrogenase [NAD(+)](EC:1.1.1.-; EC:1.1.1.232; Prostaglandin dehydrogenase 1; Short chain dehydrogenase/reductase family 36C member 1; PGDH1; SDR36C1; PGDH; PGDH1; PHOAR1;
免疫原:KLH conjugated synthetic peptide derived from human Prostaglandin dehydrogenase 1 (55-160/266aa)
预测反应性:Bovine, Equine, Mouse, Porcine, Rabbit
分子量:29 kDa
应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P15428
Note:For research use only.





别名:PGDH_HUMAN; 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; EC:1.1.1.141; 15-PGDH; 15 PGDH; 15PGDH; Eicosanoid/docosanoid dehydrogenase [NAD(+)](EC:1.1.1.-; EC:1.1.1.232; Prostaglandin dehydrogenase 1; Short chain dehydrogenase/reductase family 36C member 1; PGDH1; SDR36C1; PGDH; PGDH1; PHOAR1;
免疫原:KLH conjugated synthetic peptide derived from human Prostaglandin dehydrogenase 1 (55-160/266aa)
预测反应性:Bovine, Equine, Mouse, Porcine, Rabbit
分子量:29 kDa
应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P15428
Note:For research use only.

Anti-HPGD Antibody (C-term) at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa, Blocking/Dilution buffer: 5% NFDM/TBST.

HPGD Antibody (C-term) (orb100318) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Fixative-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PGDH) Polyclonal Antibody, Unconjugated (orb100318) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Fixative-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PGDH) Polyclonal Antibody, Unconjugated (orb100318) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Western blot analysis of HPGD Antibody (C-term) in MDA-MB231 cell line lysates (35 ug/lane). HPGD (arrow) was detected using the purified Pab.
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HPGD Rabbit Polyclonal Antibody抗体,orb100318,biorbyt
¥3120










