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HnRNP K Rabbit Polyclonal Anti

body抗体,orb101240,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb101240
  • 英国
  • 2025年12月04日
  • IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      HnRNP K Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      HnRNP K Rabbit Polyclonal Antibody

    • 靶点

      HNRNPK

    • 浓度

      1mg/ml

    • 应用范围

      IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb101240

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human hnRNP K (201-300/463aa)

    • 规格

      50 ul

    产品描述:HnRNP K Rabbit Polyclonal Antibody

    别名:CSBP; dC stretch binding protein; FLJ41122 antibody Heterogeneous nuclear ribonucleoprotein K; hnRNP K; HNRNPK; HNRPK; HNRPK_HUMAN; Transformation up regulated nuclear protein; Transformation up-regulated nuclear protein; Transformation upregulated nuclear protein; TUNP.

    免疫原:KLH conjugated synthetic peptide derived from human hnRNP K (201-300/463aa)

    预测反应性:Bovine, Canine, Gallus, Porcine, Rabbit

    分子量:51 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P61978

    Note:For research use only.

    产品细节图片1
    25 ug total protein per lane of various lysates probed with hnRNP K polyclonal antibody, unconjugated (orb101240) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

    产品细节图片2
    Fixative-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (hnRNP K) Polyclonal Antibody, Unconjugated (orb101240) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (hnRNP K) Polyclonal Antibody, Unconjugated (orb101240) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (hnRNP K) Polyclonal Antibody, Unconjugated (orb101240) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片5
    Fixative-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (hnRNP K) Polyclonal Antibody, Unconjugated (orb101240) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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    图标文献和实验
    相关实验
    • ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

      2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986

    • Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

      dilutions for rabbit polyclonal antibodies range between 1:50 and 1:500. For double-labeling experiments, use primary antibodies raised in two different species. Antibodies, secondary Secondary antibodies are diluted in PBS containing 2% normal

    • ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION

      although other techniques can be used. The choice of immobilized antibody binding protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b . Protein G binds

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