- ￥3120
- biorbyt
- orb101689
- 英国
- 2025年12月04日
- ICC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
HRG beta 1 Rabbit Polyclonal Antibody抗体
- 抗体英文名：
HRG beta 1 Rabbit Polyclonal Antibody
- 靶点：
NRG1
- 浓度：
1mg/ml
- 应用范围：
ICC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb101689
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human Neuregulin 1 (65-150/640aa)
- 规格：
50 ul
产品描述：HRG beta 1 Rabbit Polyclonal Antibody
别名:Neuregulin 1; GGF2; Acetylcholine receptor inducing activity; ARIA; Breast cancer cell differentiation factor p45; GGF; glial growth factor; Heregulin; Heregulin beta1; heregulin, alpha(45kD, ERBB2 p185 activator); HGL; HRG; HRG beta 1A; HRG1; HRGA; NDF; neu differentiation factor; NRG1; sensory and motor neuron derived factor; SMDF; NRG1_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human Neuregulin 1 (65-150/640aa)
预测反应性:Rabbit
分子量:70 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:200-800
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q02297
Note:For research use only.
别名:Neuregulin 1; GGF2; Acetylcholine receptor inducing activity; ARIA; Breast cancer cell differentiation factor p45; GGF; glial growth factor; Heregulin; Heregulin beta1; heregulin, alpha(45kD, ERBB2 p185 activator); HGL; HRG; HRG beta 1A; HRG1; HRGA; NDF; neu differentiation factor; NRG1; sensory and motor neuron derived factor; SMDF; NRG1_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human Neuregulin 1 (65-150/640aa)
预测反应性:Rabbit
分子量:70 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:200-800
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q02297
Note:For research use only.
paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.
Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Brain (Mouse) Lysate at 40 ug, Raji Cell (Human) Lysate at 40 ug, Primary: Anti-HRG beta 1 (orb101689) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 70 kD, Observed band size: 65 kD.
Tissue/cell: MCF7 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) 1:200, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37°C for 90 minutes, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
Tissue/cell: Mouse ovary tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-HRG beta 1 Polyclonal Antibody, Unconjugated (orb101689) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/cell: U251 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) 1:200, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37°C for 90 minutes, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
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HRG beta 1 Rabbit Polyclonal Antibody抗体，orb101689，biorbyt
￥3120
