- ￥3159 - 5850
- Biorbyt
- orb6569
- 英国
- 2026年07月22日
- FC, IF, IHC-Fr, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
Phospho-CDKN1A/p21 (Thr57) Rabbit Polyclonal Antibody 抗体
- 抗体英文名：
Phospho-CDKN1A/p21 (Thr57) Rabbit Polyclonal Antibody
- 靶点：
CDKN1A
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb6569
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
详询
- 规格：
50 ul/100 ul/200 ul
| 规格： | 50 ul | 产品价格： | ￥3159.0 |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥4914.0 |
| 规格： | 200 ul | 产品价格： | ￥5850.0 |
类别: Antibodies
产品子类型: Primary Antibody
描述: Phospho-CDKN1A/p21 (Thr57) Rabbit Polyclonal Antibody
别名: CAP20; CDKN1; CIP1; MDA-6; P21; SDI1; WAF1; p21CIP1; CDKI; mda6; p21WAF; UV96; CDN1A_HUMAN; CDKN1A; CDK-interacting protein 1; Melanoma differentiation-associated protein 6 (MDA-6); PIC1; CDN1A_MOUSE; Melanoma differentiation-associated protein; cyclin dependent kinase inhibitor 1A; cyclin-dependent kinase inhibitor 1A (p21, Cip1); CDKN1A (phospho-T57); p-CDKN1A; phospho-CDKN1A; CDKN1A (phospho-Thr57)
应用稀释比例: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
免疫原: KLH conjugated Synthesised phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr57 TE(p-T)PL
分子量: 18 kDa
预测反应性: Mouse, Rat
防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化方法: Affinity purified by Protein A
RRID: AB_10922310
研究领域: Signal Transduction
UniProt ID: P38936
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: Phospho-CDKN1A/p21 (Thr57) Rabbit Polyclonal Antibody
别名: CAP20; CDKN1; CIP1; MDA-6; P21; SDI1; WAF1; p21CIP1; CDKI; mda6; p21WAF; UV96; CDN1A_HUMAN; CDKN1A; CDK-interacting protein 1; Melanoma differentiation-associated protein 6 (MDA-6); PIC1; CDN1A_MOUSE; Melanoma differentiation-associated protein; cyclin dependent kinase inhibitor 1A; cyclin-dependent kinase inhibitor 1A (p21, Cip1); CDKN1A (phospho-T57); p-CDKN1A; phospho-CDKN1A; CDKN1A (phospho-Thr57)
应用稀释比例: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
免疫原: KLH conjugated Synthesised phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr57 TE(p-T)PL
分子量: 18 kDa
预测反应性: Mouse, Rat
防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化方法: Affinity purified by Protein A
RRID: AB_10922310
研究领域: Signal Transduction
UniProt ID: P38936
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Blank control: 293T. Primary Antibody (green line): Rabbit Anti-phospho-CDKN1A/p21 (Thr57) antibody (orb6569), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: human colon carcinoma, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-CDKN1A/P21 (Thr57) Polyclonal Antibody, Unconjugated (orb6569) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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文献和实验该产品被引用文献
Lehmann, Kuno et al. Liver failure after extended hepatectomy in mice is mediated by a p21-dependent barrier to liver regeneration Gastroenterology, 143, 1609-1619.e4 (2012)
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Phospho-CDKN1A/p21 (Thr57) Rabbit Polyclonal Antibody 抗体，orb6569，Biorbyt
￥3159 - 5850
