产品封面图
文献支持

14-3-3 epsilon Rabbit Polyclon

al Antibody抗体,orb6357,biorbyt
收藏
  • ¥3120
  • biorbyt已认证
  • orb6357
  • 英国
  • 2025年12月04日
  • IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
avatar
品牌商
13钻石会员
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      14-3-3 epsilon Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      14-3-3 epsilon Rabbit Polyclonal Antibody

    • 靶点

      YWHAE

    • 浓度

      1mg/ml

    • 应用范围

      IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb6357

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human 14-3-3E (151-255/255aa)

    • 规格

      50 ul

    产品描述:14-3-3 epsilon Rabbit Polyclonal Antibody

    别名:14-3-3 epsilon; 14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 E; 14-3-3 protein epsilon; 14-3-3E; 1433E_HUMAN; KCIP 1; KCIP-1; KCIP1; MDCR; MDS; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; Protein kinase C inhibitor protein1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5-monooxygenase activation protein epsilon polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide; YWHAE.

    免疫原:KLH conjugated synthetic peptide derived from human 14-3-3E (151-255/255aa)

    预测反应性:Bovine, Gallus, Rabbit, Sheep

    分子量:29 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:28835462

    UniProt ID:P62258

    Note:For research use only.

    产品细节图片1
    paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (orb6357) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片2
    Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (orb6357) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Sample: A431 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-14-3-3 epsilon (orb6357) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.

    产品细节图片4
    Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Placenta tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Testis tissue lysates, Lane 5: Rat Placenta tissue lysates, Lane 6: Human 293T cell lysates, Lane 7: Human A375 cell lysates, Lane 8: Human SW480 cell lysates, Primary: Anti-14-3-3 epsilon (orb6357) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kDa, Observed band size: 30 kDa.

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    该产品被引用文献
    A novel Pyrin-Associated Autoinflammation with Neutrophilic Dermatosis mutation further defines 14-3-3 binding of pyrin and distinction to Familial Mediterranean Fever Ann Rheum Dis, 76, 2085-2094 (2017)
    相关实验
    • 14-3-3 proteins

      of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins. A polyclonal antibody was raised against purified ST-IV. A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti

    • Introduction of human 14-3-3 proteins

      of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins.A polyclonal antibody was raised against purified ST-IV.A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti

    • ALKALINE PHOSPHATASE (APAAP) TECHNIQUE

      antibody. 30 minutes. 5. Jet wash in tris/HC1 saline buffer pH 7.6. 6. Wash in buffer. 3 minutes. Steps 7-9 for POLYCLONAL Primary Antisera Only 7. Incubate in mouse anti-rabbit immunoglobulins. 30 minutes. 8. Jet wash in buffer.

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    ¥1500
    爱必信(上海)生物科技有限公司
    2025年07月15日询价
    ¥5530
    上海瑶韵生物科技有限公司
    2026年03月23日询价
    ¥1243
    武汉博欧特生物科技有限公司
    2025年07月07日询价
    ¥3885
    北京智杰方远科技有限公司
    2025年07月09日询价
    询价
    赛默飞世尔科技
    2023年10月17日询价
    文献支持
    14-3-3 epsilon Rabbit Polyclonal Antibody抗体,orb6357,biorbyt
    ¥3120