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Phospho-IRAK1 (Thr387) Rabbit

Polyclonal Antibody抗体,orb6223,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb6223
  • 英国
  • 2025年12月04日
  • ELISA, FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Phospho-IRAK1 (Thr387) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-IRAK1 (Thr387) Rabbit Polyclonal Antibody

    • 靶点

      Irak1

    • 浓度

      1mg/ml

    • 应用范围

      ELISA, FC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb6223

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr387 RG(p-T)LA

    • 规格

      50 ul

    产品描述:Phospho-IRAK1 (Thr387) Rabbit Polyclonal Antibody

    别名:IRAK(phospho T387); p-IRAK(phospho T387); IRAK1(phospho-Thr387); IRAK1(phospho-T387); IRAK1(p-Thr387); Il1rak; Il1rak; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 2; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; IRAK1; IRAK1; IRAK1_HUMAN; IRAK2; IRAK2; mPLK; mPLK; Pelle; Pelle; Pelle homolog; Pelle-like protein kinase; Plpk.

    免疫原:KLH conjugated Synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr387 RG(p-T)LA

    预测反应性:Bovine, Canine, Porcine, Rabbit

    分子量:78 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /Test, ELISA=1:5000-10000

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:24161715

    UniProt ID:P51617

    Note:For research use only.

    产品细节图片1
    Blank control (blue): RSC96 cells (fixed with 2% Fixative (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-IRAK1 (Thr387) antibody (orb6223), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

    产品细节图片2
    Fixative-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRAK1 (Thr387)) Polyclonal Antibody, Unconjugated (orb6223) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-IRAK1 (Thr387)) Polyclonal Antibody, Unconjugated (orb6223) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRAK1 (Thr387)) Polyclonal Antibody, Unconjugated (orb6223) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片5
    phosphopeptide, non phosphopeptide.

    产品细节图片6
    Sample: Lane 1: Liver (Mouse) Cell Lysate at 30 ug, Lane 2: Colon carcinoma (Human) Lysate at 30 ug, Primary: Anti-Phospho-IRAK1 (Thr387) (orb6223) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 70kD, Observed band size: 70kD.

    产品细节图片7
    Tissue/Cell: mouse spleen tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-IRAK1 (Thr387) Polyclonal Antibody, Unconjugated (orb6223) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
    该产品被引用文献
    Summers, Lauren et al. Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury Mol Cell Neurosci, 57, 93-103 (2013)
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    Phospho-IRAK1 (Thr387) Rabbit Polyclonal Antibody抗体,orb6223,biorbyt
    ¥3120