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G6PC Rabbit Polyclonal Antibod

y抗体,orb6097,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb6097
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      G6PC Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      G6PC Rabbit Polyclonal Antibody

    • 靶点

      G6PC

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb6097

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human Glucose 6 phosphatase alpha (81-180/357aa)

    • 规格

      50 ul

    产品描述:G6PC Rabbit Polyclonal Antibody

    别名:glucose-6-phosphatase, catalytic subunit; GSD1; AW107337; G-6-Pase; G6Pase; G6Pase-alpha; Glucose 6 phosphatase alpha; G6PC_HUMAN; G6PT; Glucose-6-phosphatase alpha; Glucose-6-phosphatase; GSD1a; MGC163350; MGC93613; RP23-281C18.19.

    免疫原:KLH conjugated synthetic peptide derived from human Glucose 6 phosphatase alpha (81-180/357aa)

    预测反应性:Bovine, Canine, Mouse, Porcine, Rabbit, Rat, Sheep

    分子量:39 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:27914922, 30496623

    UniProt ID:P35575

    Note:For research use only.

    产品细节图片1
    paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Glucose 6 phosphatase alpha) Polyclonal Antibody, Unconjugated (orb6097) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片2
    Sample: Lane 1: Human Hela cell lysates, Lane 2: Human U937cell lysates, Lane 3: Human HL-60 cell lysates, Lane 4: Human HepG2 cell lysates, Lane 5: Human SH-SY5Y cell lysates, Primary: Anti-Glucose 6 phosphatase alpha (orb6097) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kDa, Observed band size: 35 kDa.

    产品细节图片3
    U-937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with orb6097 Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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    图标文献和实验
    该产品被引用文献
    Mizuno, Tooru M. et al. Negative regulation of hepatic fat mass and obesity associated (Fto) gene expression by insulin Life Sci, 170, 50-55 (2017), Zhang, Mian et al. Prevention of Injury-Induced Osteoarthritis in Rodent Temporomandibular Joint by Targeting Chondrocyte CaSR J Bone Miner Res, 34, 726-738 (2019)
    相关实验
    • Polyclonal Antibody Production

      Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed        

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Preparation of Polyclonal Antibodies to Retinoid Receptors

      rabbit-antibody production and affinity purification. Suggestions for potential applications in the study of retinoid-molecular biology are also discussed.

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    文献支持
    G6PC Rabbit Polyclonal Antibody抗体,orb6097,biorbyt
    ¥3120