- ￥3120
- biorbyt
- orb5930
- 英国
- 2025年12月04日
- FC
- Rabbit
- Human
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- 详细信息
- 技术资料
- 抗体名：
Phospho-Cyclin E1 (Thr77) Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Phospho-Cyclin E1 (Thr77) Rabbit Polyclonal Antibody
- 靶点：
CCNE1
- 浓度：
1mg/ml
- 应用范围：
FC
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb5930
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77 IP(p-T)PD
- 规格：
50 ul
产品描述：Phospho-Cyclin E1 (Thr77) Rabbit Polyclonal Antibody
别名:Cyclin E1(phospho-Thr77); Cyclin E1(phospho-T77); CCNE 1; CCNE; CCNE1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1; CCNE1_HUMAN; pCCNE1.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77 IP(p-T)PD
预测反应性:Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat
分子量:47 kDa
应用稀释比例：Flow-Cyt=1μg /Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P24864
Note:For research use only.
别名:Cyclin E1(phospho-Thr77); Cyclin E1(phospho-T77); CCNE 1; CCNE; CCNE1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1; CCNE1_HUMAN; pCCNE1.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77 IP(p-T)PD
预测反应性:Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat
分子量:47 kDa
应用稀释比例：Flow-Cyt=1μg /Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P24864
Note:For research use only.
Blank control (blue line): MCF7 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-phospho-Cyclin E1 (Thr77) antibody (orb5930), dilution: 3 µg/10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. The cells were Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-Cyclin E1 (Thr77) antibody, dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-phospho-Cyclin E1 (Thr77) antibody (orb5930), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
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Phospho-Cyclin E1 (Thr77) Rabbit Polyclonal Antibody抗体，orb5930，biorbyt
￥3120
