- ￥3120
- biorbyt
- orb5735
- 英国
- 2025年12月04日
- ELISA, FC, ICC, IF, IHC-Fr, IHC-P
- Rabbit
- Human, Rabbit, Rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 抗体名：
Phospho-ATG1 (Ser556) Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Phospho-ATG1 (Ser556) Rabbit Polyclonal Antibody
- 靶点：
ULK1
- 浓度：
1mg/ml
- 应用范围：
ELISA, FC, ICC, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human, Rabbit, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb5735
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human ATG1/ULK1 around the phosphorylation site of Ser556 LH(p-S)AP
- 规格：
50 ul
产品描述：Phospho-ATG1 (Ser556) Rabbit Polyclonal Antibody
别名:ULK1(phospho S556); ULK1(phospho Ser556); p-ULK1(Ser556); ATG 1; ULK1; ATG1; ATG1A; Serine/threonine protein kinase ULK1; Serine/threonine protein kinase Unc51.1; ULK 1; Unc 51(C. elegans) like kinase 1; UNC 51; Unc 51 like kinase 1; Unc-51-like kinase 1; Unc-51 like kinase 1(C. elegans); UNC51; ULK1_HUMAN; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; hATG1.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human ATG1/ULK1 around the phosphorylation site of Ser556 LH(p-S)AP
预测反应性:Equine, Mouse, Porcine
分子量:116 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=3ug/test, ELISA=1:5000-10000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:O75385
Note:For research use only.
别名:ULK1(phospho S556); ULK1(phospho Ser556); p-ULK1(Ser556); ATG 1; ULK1; ATG1; ATG1A; Serine/threonine protein kinase ULK1; Serine/threonine protein kinase Unc51.1; ULK 1; Unc 51(C. elegans) like kinase 1; UNC 51; Unc 51 like kinase 1; Unc-51-like kinase 1; Unc-51 like kinase 1(C. elegans); UNC51; ULK1_HUMAN; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; hATG1.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human ATG1/ULK1 around the phosphorylation site of Ser556 LH(p-S)AP
预测反应性:Equine, Mouse, Porcine
分子量:116 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=3ug/test, ELISA=1:5000-10000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:O75385
Note:For research use only.
Blank control: A549. Primary Antibody (green line): Rabbit Anti-Phospho-ATG1 (Ser556) antibody (orb5735), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-ATG1 (Ser556)) polyclonal Antibody, Unconjugated (orb5735) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
phosphopeptide, non phosphopeptide.
Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-ATG1 (Ser556) Polyclonal Antibody, Unconjugated (orb5735) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
Phospho-ATG1 (Ser556) Rabbit Polyclonal Antibody抗体，orb5735，biorbyt
￥3120
