BACH1 Rabbit Polyclonal Antibody抗体,orb4401,biorbyt

BACH1 Rabbit Polyclonal Antibo

dy抗体,orb4401,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb4401
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      BACH1 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      BACH1 Rabbit Polyclonal Antibody

    • 靶点

      BACH1

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb4401

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human BACH1 (501-600/736aa)

    • 规格

      50 ul

    产品描述:BACH1 Rabbit Polyclonal Antibody

    别名:BTB Domain And CNC Homolog 1; BTB And CNC Homology 1, Basic Leucine Zipper Transcription Factor 1; Basic Region Leucine Zipper Transcriptional Regulator BACH1; Transcription Regulator Protein BACH1; BTB And CNC Homolog 1; BACH-1; BTBD24; HA2303; BACH1_HUMAN;

    免疫原:KLH conjugated synthetic peptide derived from human BACH1 (501-600/736aa)

    预测反应性:Bovine, Canine, Equine, Gallus, Porcine, Rabbit

    分子量:81 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:O14867

    Note:For research use only.

    BACH1 Rabbit Polyclonal Antibo
    Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-BACH1 antibody (orb4401), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    BACH1 Rabbit Polyclonal Antibo
    Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-BACH1 (orb4401) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

    BACH1 Rabbit Polyclonal Antibo
    Tissue/Cell: Rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-BACH1 Polyclonal Antibody, Unconjugated (orb4401) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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