Phospho-c-Abl (Tyr283) Rabbit Polyclonal Antibody抗体,orb4326,biorbyt

Phospho-c-Abl (Tyr283) Rabbit

Polyclonal Antibody抗体,orb4326,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb4326
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      Phospho-c-Abl (Tyr283) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-c-Abl (Tyr283) Rabbit Polyclonal Antibody

    • 靶点

      ABL1

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb4326

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human c-Abl KK(p-Y)SL

    • 规格

      50 ul

    产品描述:Phospho-c-Abl (Tyr283) Rabbit Polyclonal Antibody

    别名:c-Abl(phospho Y238); c-Abl(phospho Tyr238); p-c-Abl(Tyr238); tyrosine-protein kinase ABL1 isoform b; Abelson Murine Leukemia Viral Oncogene Homolog 1; Abelson murine leukemia viral v abl oncogene homolog 1; Abl 1; ABL; Abl protein; Abl1; Bcr/c abl oncogene protein; JTK 7; JTK7; p150; Proto oncogene tyrosine protein kinase ABL1; Transformation gene oncogene ABL; v abl Abelson murine leukemia viral oncogene homolog 1; v abl; ABL1_HUMAN.

    免疫原:KLH conjugated synthetic peptide derived from human c-Abl KK(p-Y)SL

    预测反应性:Bovine, Canine, Equine, Mouse, Porcine, Rabbit

    分子量:124 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P00519

    Note:For research use only.

    Phospho-c-Abl (Tyr283) Rabbit
    Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-phospho-c-Abl (Tyr283) antibody (orb4326), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    Phospho-c-Abl (Tyr283) Rabbit
    Fixative-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-Abl (Tyr283)) Polyclonal Antibody, Unconjugated (orb4326) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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