- ￥3120
- biorbyt
- orb100267
- 英国
- 2025年12月04日
- FC, IF, IHC-Fr, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
Phospho-Histone H1.4 (Thr18) Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Phospho-Histone H1.4 (Thr18) Rabbit Polyclonal Antibody
- 靶点：
H1-4
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb100267
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
参考产品说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18 EK(p-T)P
- 规格：
50 ul
产品描述：Phospho-Histone H1.4 (Thr18) Rabbit Polyclonal Antibody
别名:Histone H1.4(phospho T18); p-Histone H1.4(phospho T18); HIST1H1E; H1F4; Histone H1.4; Histone H1b; H14_HUMAN; Histone H1s-4; H1 histone family member 4; H1E; Hist1h1e; Histone 1 H1e; Histone cluster 1 H1e; Histone H1; MGC116819; H1.4; H1F0; Histone H1b.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18 EK(p-T)P
预测反应性:Mouse, Rat
分子量:22 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P10412
Note:For research use only.
别名:Histone H1.4(phospho T18); p-Histone H1.4(phospho T18); HIST1H1E; H1F4; Histone H1.4; Histone H1b; H14_HUMAN; Histone H1s-4; H1 histone family member 4; H1E; Hist1h1e; Histone 1 H1e; Histone cluster 1 H1e; Histone H1; MGC116819; H1.4; H1F0; Histone H1b.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18 EK(p-T)P
预测反应性:Mouse, Rat
分子量:22 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P10412
Note:For research use only.
Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-phospho-Histone H1.4 (Thr18) antibody (orb100267), Dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (orb100267) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (orb100267) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (orb100267) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (orb100267) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-phospho-Histone H1.4 (Thr18) Polyclonal Antibody, Unconjugated (orb100267) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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Phospho-Histone H1.4 (Thr18) Rabbit Polyclonal Antibody抗体，orb100267，biorbyt
￥3120
