- ￥3120
- biorbyt
- orb13631
- 英国
- 2025年12月04日
- WB
- Rabbit
- Human
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- 详细信息
- 技术资料
- 抗体名：
P53 (wt-p53) Rabbit Polyclonal Antibody抗体
- 抗体英文名：
P53 (wt-p53) Rabbit Polyclonal Antibody
- 靶点：
TP53
- 浓度：
1mg/ml
- 应用范围：
WB
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb13631
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
参考产品说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human P53 (301-393/393aa)
- 规格：
50 ul
产品描述：P53 (wt-p53) Rabbit Polyclonal Antibody
别名:P53_HUMAN; Cellular tumor antigen p53; TP53; Antigen NY-CO-13;Phosphoprotein p53; Tumor suppressor p53; tumor protein p53; BCC7; LFS1; BMFS5; TRP53; P 53; P-53; wt-p53; Widespread p53; Wtp53; Wt p53;
免疫原：KLH conjugated synthetic peptide derived from human P53 (301-393/393aa)
预测反应性:Canine, Equine, Porcine, Rabbit, Sheep
分子量:53 kDa
应用稀释比例：WB=1:500-2000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P04637
Note:For research use only.
别名:P53_HUMAN; Cellular tumor antigen p53; TP53; Antigen NY-CO-13;Phosphoprotein p53; Tumor suppressor p53; tumor protein p53; BCC7; LFS1; BMFS5; TRP53; P 53; P-53; wt-p53; Widespread p53; Wtp53; Wt p53;
免疫原：KLH conjugated synthetic peptide derived from human P53 (301-393/393aa)
预测反应性:Canine, Equine, Porcine, Rabbit, Sheep
分子量:53 kDa
应用稀释比例：WB=1:500-2000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P04637
Note:For research use only.
Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-P53 (wt-p53) antibody (orb13631), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Liver (Mouse) Lysate at 40 ug, Liver (Rat) Lysate at 40 ug, Primary: Anti-P53 (wt-p53) (orb13631) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.
Sample: MCF-7 (Human) Cell Lysate at 40 ug, DU145 (Human) Cell Lysate at 40 ug, Primary: Anti-P53 (wt-p53) (orb13631) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.
Tissue/Cell: A549 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (P53 (wt-p53)) polyclonal Antibody, Unconjugated (orb13631) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: A549 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (P53 (wt-p53)) polyclonal Antibody, Unconjugated (orb13631) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-P53 (wt-p53) Polyclonal Antibody, Unconjugated (orb13631) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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P53 (wt-p53) Rabbit Polyclonal Antibody抗体，orb13631，biorbyt
￥3120
