- ￥3120
- biorbyt
- orb13279
- 英国
- 2025年12月04日
- FC, ICC, IF, IHC-Fr, IHC-P
- Rabbit
- Human, Rat
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- 详细信息
- 技术资料
- 抗体名：
Phospho-beta catenin (Tyr142) Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Phospho-beta catenin (Tyr142) Rabbit Polyclonal Antibody
- 靶点：
CTNNB1
- 浓度：
1mg/ml
- 应用范围：
FC, ICC, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb13279
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
参考产品说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human Beta catenin around the phosphorylation site of Tyr142 IN(p-Y)QD
- 规格：
50 ul
产品描述：Phospho-beta catenin (Tyr142) Rabbit Polyclonal Antibody
别名:CTNB1_HUMAN; Catenin beta-1; catenin beta 1; CTNNB1; Catenin beta; Beta-catenin; CTNNB; OK/SW-cl.35; PRO2286; EVR7; MRD19; NEDSDV; armadillo; βcatenin; β catenin; β-catenin;
免疫原：KLH conjugated Synthesised phosphopeptide derived from human Beta catenin around the phosphorylation site of Tyr142 IN(p-Y)QD
预测反应性:Gallus, Mouse
分子量:86 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P35222
Note:For research use only.
别名:CTNB1_HUMAN; Catenin beta-1; catenin beta 1; CTNNB1; Catenin beta; Beta-catenin; CTNNB; OK/SW-cl.35; PRO2286; EVR7; MRD19; NEDSDV; armadillo; βcatenin; β catenin; β-catenin;
免疫原：KLH conjugated Synthesised phosphopeptide derived from human Beta catenin around the phosphorylation site of Tyr142 IN(p-Y)QD
预测反应性:Gallus, Mouse
分子量:86 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P35222
Note:For research use only.
Blank control: Hela (fixed with 2% Fixative (10 min), then permeabilized with 0.3% tritionx-100 for 5 min at room temperature). Primary Antibody: Rabbit Anti-phospho-beta catenin (Tyr142) antibody (orb13279, Green), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-FITC), Dilution: 1:200 in 1X PBS containing 0.5% BSA.
Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-beta catenin (Tyr142)) polyclonal Antibody, Unconjugated (orb13279) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-beta catenin (Tyr142) Polyclonal Antibody, Unconjugated (orb13279) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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Phospho-beta catenin (Tyr142) Rabbit Polyclonal Antibody抗体，orb13279，biorbyt
￥3120
