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E cadherin Rabbit Polyclonal A

ntibody (FITC)抗体，orb15536，biorbyt
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  • ￥7020
  • biorbyt已认证
  • orb15536
  • 英国
  • 2025年12月04日
  • FC
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      E cadherin Rabbit Polyclonal Antibody (FITC)抗体

    • 抗体英文名：

      E cadherin Rabbit Polyclonal Antibody (FITC)

    • 靶点：

      CDH1

    • 浓度：

      1mg/ml

    • 应用范围：

      FC

    • 宿主：

      Rabbit

    • 适应物种：

      Human

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb15536

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      FITC

    • 克隆性：

      Polyclonal

    • 保存条件：

      参考产品说明书

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from human E-cadherin (841-882/882aa)

    • 规格：

      100 ul

    产品描述：E cadherin Rabbit Polyclonal Antibody (FITC)

    别名:E-cadherin; anion exchanger protein 3; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; CADH1_HUMAN.

    免疫原：KLH conjugated synthetic peptide derived from human E-cadherin (841-882/882aa)

    预测反应性:Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rat

    应用稀释比例：Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Pubmed:25450039, 33945102

    Note:For research use only.

    产品细节图片1
    Blank control: A431. Primary Antibody (green line): Rabbit Anti-E cadherin antibody (orb15536), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-E cadherin antibody (orb15536), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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    图标文献和实验
    该产品被引用文献
    Pal, Sumanta K. et al. Detection and phenotyping of circulating tumor cells in high-risk localized prostate cancer Clin Genitourin Cancer, 13, 130-136 (2015), K, Curzytek et al. Immune-Regulatory and Molecular Effects of Antidepressants on the Inflamed Human Keratinocyte HaCaT Cell Line Neurotox Res, (2021)
    相关实验

    • Antibody Conjugation Protocol

      /P ratio: = moles of FITC/moles of protein g) F/P ratio should be between 5:1 and 6:1 for Flow Cytometric use.   上一篇：Testing Rabbit Bleeds By Elisa   下一篇：monoclonal antibody production fusion

    • ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

      from different species and for different isotypes within a species. Tables I may be used to determine relative avidity for polyclonal antibodies of a given species. Table II shows relative affinities for varius isotypes.                           

    • secondary antibody review -- data from 99 publications

      localization of tagged SAX-3 in 293T cell Molecular Probes 2       Alexa Fluor 647 immunocytochemistry   localization of BCG phagosomes   3       Alexa Fluor 647 immunohistochemistry   detect surface DFz2   3       FITC immunohistochemistry   determine

    查看更多相关实验 >
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    E cadherin Rabbit Polyclonal Antibody (FITC)抗体，orb15536，biorbyt
    ￥7020