- ￥3120
- biorbyt
- orb11559
- 英国
- 2025年12月04日
- FC, ICC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Vimentin Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Vimentin Rabbit Polyclonal Antibody
- 靶点：
VIM
- 浓度：
1mg/ml
- 应用范围：
FC, ICC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb11559
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
参考产品说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human Vimentin (371-466/466aa)
- 规格：
50 ul
产品描述：Vimentin Rabbit Polyclonal Antibody
别名:FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.
免疫原：KLH conjugated synthetic peptide derived from human Vimentin (371-466/466aa)
预测反应性:Bovine, Gallus, Goat, Porcine
分子量:53 kDa
应用稀释比例：WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:100-500, IF=1:200-1000, Flow-Cyt=1μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:40537738
UniProt ID:P08670
Note:For research use only.
别名:FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.
免疫原：KLH conjugated synthetic peptide derived from human Vimentin (371-466/466aa)
预测反应性:Bovine, Gallus, Goat, Porcine
分子量:53 kDa
应用稀释比例：WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:100-500, IF=1:200-1000, Flow-Cyt=1μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:40537738
UniProt ID:P08670
Note:For research use only.
Blank control: A549. Primary Antibody (green line): Rabbit Anti-Vimentin antibody (orb11559), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Jurkat cells (blue). Primary Antibody: Rabbit Anti-Vimentin antibody antibody (orb11559), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% Fixative (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (orb11559, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb11559) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb11559) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Fixative-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb11559) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Fixative-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb11559) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb11559) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb11559) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb11559) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb11559) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
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文献和实验该产品被引用文献
Ahmed M Abou-Shanab # 1 2, Shaimaa Shouman # 1 2, Alaa E Hussein 1 2, Ola A Gaser 1 2, Shireen Magdy 1 2, Eman Ashraf 1 2, Radwa Ayman Salah 1 2, Omaima Idris 3, Nagwa El-Badri Amniotic membrane promotes doxorubicin potency by suppressing SH-SY5Y neuroblastoma cell angiogenesis BMC Cance, (2025)
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Vimentin Rabbit Polyclonal Antibody抗体，orb11559，biorbyt
￥3120
