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MAP126 Rabbit Polyclonal Antib

ody抗体,orb11404,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb11404
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      MAP126 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      MAP126 Rabbit Polyclonal Antibody

    • 靶点

      SPAG5

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb11404

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human SPAG5 (1101-1193/1193aa)

    • 规格

      50 ul

    产品描述:MAP126 Rabbit Polyclonal Antibody

    别名:Astrin; Deepest; hMAP 126; hMAP126; MAP 126; Mitotic spindle associated protein; Mitotic spindle associated protein p126; Mitotic spindle coiled coil related protein; SPAG 5; SPAG5; Sperm associated antigen 5; Sperm tail protein Spag 5; Sperm tail protein Spag5; SPAG5_HUMAN.

    免疫原:KLH conjugated synthetic peptide derived from human SPAG5 (1101-1193/1193aa)

    预测反应性:Bovine, Canine, Equine, Mouse, Porcine

    分子量:134 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q96R06

    Note:For research use only.

    产品细节图片1
    Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-MAP126 antibody (orb11404), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MAP126) polyclonal Antibody, Unconjugated (orb11404) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片3
    Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SPAG5/MAP126/Astrin Polyclonal Antibody, Unconjugated (orb11404) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

    产品细节图片4
    Tissue/Cell: rat testis tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SPAG5/MAP126/Astrin Polyclonal Antibody, Unconjugated (orb11404) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
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