- ￥3120
- biorbyt
- orb10803
- 英国
- 2025年12月04日
- FC, ICC, IF, IHC-Fr, IHC-P
- Rabbit
- Human, Mouse
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- 详细信息
- 技术资料
- 抗体名：
HIF1 beta Rabbit Polyclonal Antibody抗体
- 抗体英文名：
HIF1 beta Rabbit Polyclonal Antibody
- 靶点：
ARNT
- 浓度：
1mg/ml
- 应用范围：
FC, ICC, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human, Mouse
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb10803
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
参考产品说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human HIF-1 Beta (681-789/789aa)
- 规格：
50 ul
产品描述：HIF1 beta Rabbit Polyclonal Antibody
别名:HIF1 beta; ARNT1; ARNT; HIF 1 beta; HIF-1 beta; ARNT protein; Aryl Hydrocarbon Receptor Nuclear Translocator; Dioxin Receptor; Dioxin receptor nuclear translocator; Drnt; HIF 1 beta; HIF 1beta; HIF1B; HIF1beta; Hypoxia Inducible Factor 1; Hypoxia inducible factor 1 beta; Tango; ARNT_HUMAN; Class E basic helix-loop-helix protein 2; bHLHe2; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta; HIF-1 β; HIF1-β; HIF 1-β; HIF-1-β; HIF 1 β; HIF1β; HIF1 β; HIF1-β; HIF 1β.
免疫原：KLH conjugated synthetic peptide derived from human HIF-1 Beta (681-789/789aa)
预测反应性:Bovine, Canine, Equine, Porcine, Rabbit, Rat
分子量:87 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P27540
Note:For research use only.
别名:HIF1 beta; ARNT1; ARNT; HIF 1 beta; HIF-1 beta; ARNT protein; Aryl Hydrocarbon Receptor Nuclear Translocator; Dioxin Receptor; Dioxin receptor nuclear translocator; Drnt; HIF 1 beta; HIF 1beta; HIF1B; HIF1beta; Hypoxia Inducible Factor 1; Hypoxia inducible factor 1 beta; Tango; ARNT_HUMAN; Class E basic helix-loop-helix protein 2; bHLHe2; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta; HIF-1 β; HIF1-β; HIF 1-β; HIF-1-β; HIF 1 β; HIF1β; HIF1 β; HIF1-β; HIF 1β.
免疫原：KLH conjugated synthetic peptide derived from human HIF-1 Beta (681-789/789aa)
预测反应性:Bovine, Canine, Equine, Porcine, Rabbit, Rat
分子量:87 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P27540
Note:For research use only.
Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-HIF1 beta antibody (orb10803), Dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
HepG2 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HIF1 beta) polyclonal Antibody, Unconjugated (orb10803) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Fixative-fixed, paraffin embedded (Mouse uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HIF1 beta) Polyclonal Antibody, Unconjugated (orb10803) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
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HIF1 beta Rabbit Polyclonal Antibody抗体，orb10803，biorbyt
￥3120
