- ￥3120
- biorbyt
- orb10203
- 英国
- 2025年12月04日
- ELISA, IF, IHC-Fr, IHC-P
- Rabbit
- Human, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
BRCA2 Rabbit Polyclonal Antibody抗体
- 抗体英文名：
BRCA2 Rabbit Polyclonal Antibody
- 靶点：
BRCA2
- 浓度：
1mg/ml
- 应用范围：
ELISA, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb10203
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
参考产品说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human BRCA2 (21-130/3418aa)
- 规格：
50 ul
产品描述：BRCA2 Rabbit Polyclonal Antibody
别名:BRCA2_HUMAN; Breast cancer type 2 susceptibility protein; FACD; FANCD1; Fanconi anemia group D1 protein; BRCA 2; BRCA2 DNA repair associated; FAD; FAD1; GLM3; BRCC2; FANCD; PNCA2; XRCC11; BROVCA2;
免疫原：KLH conjugated synthetic peptide derived from human BRCA2 (21-130/3418aa)
预测反应性:Mouse
分子量:384 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:29497044
UniProt ID:P51587
Note:For research use only.
别名:BRCA2_HUMAN; Breast cancer type 2 susceptibility protein; FACD; FANCD1; Fanconi anemia group D1 protein; BRCA 2; BRCA2 DNA repair associated; FAD; FAD1; GLM3; BRCC2; FANCD; PNCA2; XRCC11; BROVCA2;
免疫原：KLH conjugated synthetic peptide derived from human BRCA2 (21-130/3418aa)
预测反应性:Mouse
分子量:384 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:29497044
UniProt ID:P51587
Note:For research use only.
paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BRCA2) Polyclonal Antibody, Unconjugated (orb10203) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (Rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BRCA2) Polyclonal Antibody, Unconjugated (orb10203) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BRCA2) Polyclonal Antibody, Unconjugated (orb10203) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: MCF-7 (Human) Cell Lysate at 30 ug, CAPAN1 (Human) Cell Lysate at 30 ug, Primary: Anti-BRCA2 (orb10203) at 1/200 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 384 kD, Observed band size: 384 kD.
Tissue/cell: human breast carcinoma, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-BRCA2/FANCB Polyclonal Antibody, Unconjugated (orb10203) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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文献和实验该产品被引用文献
Goffena, Joy et al. Elongator and codon bias regulate protein levels in mammalian peripheral neurons Nat Commun, 9, 889 (2018)
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BRCA2 Rabbit Polyclonal Antibody抗体，orb10203，biorbyt
￥3120
