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PDGF-B Rabbit Polyclonal Antib

ody抗体,orb11254,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb11254
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      PDGF-B Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      PDGF-B Rabbit Polyclonal Antibody

    • 靶点

      PDGFB

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb11254

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human PDGF-B (155-210/241aa)

    • 规格

      50 ul

    产品描述:PDGF-B Rabbit Polyclonal Antibody

    别名:PDGF beta; PDGF-B; Platelet-derived growth factor B-chain; PDGF AB+BB; Becaplermin; c sis; FLJ1285; Oncogene SIS; PDGF 1; PDGF 2; PDGF A; PDGF A chain; PDGF B chain; PDGF subunit A; PDGF subunit B; PDGF1; PDGF2; PDGFA; PDGFB; PDGFR1; Platelet derived growth factor 2; Platelet derived growth factor alpha; Platelet derived growth factor alpha chain; Platelet derived growth factor alpha polypeptide; Platelet derived growth factor B chain; Platelet derived growth factor beta; Platelet derived growth factor beta polypeptide; Platelet derived growth factor subunit A; Platelet derived growth factor subunit B; SIS; SSV; v sis platelet derived growth factor beta polypeptide.

    免疫原:KLH conjugated synthetic peptide derived from human PDGF-B (155-210/241aa)

    预测反应性:Bovine, Canine, Equine, Rabbit, Sheep

    分子量:27 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P01127

    Note:For research use only.

    产品细节图片1
    Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-PDGF-B antibody (orb11254), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): F (ab')2 fragment goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 2% Fixative (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: Lung (Mouse) Lysate at 40 ug, Lane 5: Lung (Rat) Lysate at 40 ug, Lane 6: Huvec (Human) Cell Lysate at 30 ug, Lane 7: A431 (Human) Cell Lysate at 30 ug, Lane 8: MCF-7 (Human) Cell Lysate at 30 ug, Lane 9: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-PDGF-B (orb11254) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 28 kD.

    产品细节图片3
    Sample: Stomach (Rat) Lysate at 40 ug, Primary: Anti-PDGF-B (orb11254) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 32 kD.

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    图标文献和实验
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