吡`咯啉-5-羧酸合成酶试剂盒，用于样本中P5CS活性检测，

CheKine™ 吡`咯啉-5-羧酸合成酶（P5CS）活性检测试剂盒（微量法）

货号：KTB3014-S

产品概述

CheKine™ 吡`咯啉-5-羧酸合成酶（P5CS）活性检测试剂盒（微量法）是一款基于微量法原理，用于精确检测植物组织中P5CS酶活性的核心产品。P5CS是植物体内脯氨酸生物合成途径中的关键酶，对植物响应逆境胁迫具有重要调控作用。本试剂盒通过钼酸铵比色法，测定单位时间内无机磷的生成量，从而准确反映P5CS的催化活性。

检测原理

在该酶促反应体系中，P5CS催化L-谷氨酸生成L-吡`咯啉-5-羧酸（P5C），并伴随ATP分解产生ADP与无机磷。通过加入钼酸铵显色试剂，与反应生成的无机磷进行特异性结合，在660 nm波长下测定吸光度值的变化，进而计算出P5CS的酶活性水平。

产品特点与优势

• 采用微量法检测，样本需求量少，节约珍贵样本
• 经过多种植物组织样本验证，适用性广泛
• 操作流程简便高效，实验周期短
• 试剂盒提供标准品，便于定量分析与结果校准

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent III
• Reagent IV
• Reagent V
• Standard

适用样本类型

本试剂盒适用于植物组织样本，如叶片、根、茎等。推荐使用新鲜样本进行检测。若需保存，样本可在-80℃条件下存放数周。解冻过程需严格控制温度与时间，室温解冻应在4小时内完成。

保存条件与运输

试剂盒请在4℃避光条件下保存，有效期6个月。产品通过冰袋（蓝冰）运输，以确保试剂的稳定性。

注意事项

• 实验前请确保所有试剂及设备均处于适宜温度
• 操作过程中请避免反复冻融试剂
• 建议根据样本活性预实验调整样本上样量

规格与价格

规格	价格（元）	货期
48 T/48 S	516	现货（次日发货）
96 T/48 S	948	现货（次日发货）

警告与用途说明

本产品仅限用于科学研究，不得用于人类或临床诊断。使用本产品可能受特定专利或许可证限制，使用者须自行负责确保符合相关法律法规要求。对于因使用本产品可能引发的专利侵权或其他违规行为，本公司不承担任何责任。

背景信息

吡`咯啉-5-羧酸合成酶（P5CS）是植物脯氨酸合成途径中的限速酶，尤其在逆境胁迫（如干旱、盐碱、低温等）条件下，其活性上调可显著增加脯氨酸积累，增强植物的抗逆性。该试剂盒为植物抗逆生理研究提供了有效的工具。

别名：吡`咯啉-5-羧酸合成酶（P5CS）| P5CS Activity Assay Kit

检测指标：吡`咯啉-5-羧酸合成酶（P5CS）活性

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Pyroline-5-Carboxylate Synthase (P5CS) Activity Assay Kit (Micro Method)

Catalog Number: KTB3014-S

Product Overview

The CheKine™ Pyroline-5-Carboxylate Synthase (P5CS) Activity Assay Kit (Micro Method) is a core product based on the micro method principle, designed for the precise detection of P5CS enzyme activity in plant tissues. P5CS is a key enzyme in the proline biosynthesis pathway in plants and plays a significant regulatory role in plant responses to stress. This kit employs the ammonium molybdate colorimetric method to measure the amount of inorganic phosphate generated per unit time, thereby accurately reflecting the catalytic activity of P5CS.

Assay Principle

In this enzymatic reaction system, P5CS catalyzes the conversion of L-glutamate to L-Δ¹-pyrroline-5-carboxylate (P5C), accompanied by ATP hydrolysis to produce ADP and inorganic phosphate. By adding ammonium molybdate chromogenic reagent, which specifically binds to the inorganic phosphate generated in the reaction, the change in absorbance at 660 nm is measured to calculate the P5CS enzyme activity level.

Features and Advantages

• Utilizes a micro method for detection, requiring minimal sample volume and conserving precious samples
• Validated with various plant tissue samples, ensuring broad applicability
• Simple and efficient operation process with a short experimental cycle
• Includes standard for quantitative analysis and result calibration

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Standard

Applicable Sample Types

This kit is suitable for plant tissue samples, such as leaves, roots, and stems. Fresh samples are recommended for detection. If storage is necessary, samples can be kept at -80°C for several weeks. The thawing process must be strictly controlled in terms of temperature and time; thawing at room temperature should be completed within 4 hours.

Storage Conditions and Shipping

The kit should be stored at 4°C protected from light and is valid for 6 months. The product is shipped with ice packs (blue ice) to ensure reagent stability.

Precautions

• Ensure all reagents and equipment are at appropriate temperatures before starting the experiment
• Avoid repeated freeze-thaw cycles of reagents during operation
• It is recommended to adjust the sample loading volume based on pre-experimental sample activity

Specifications and Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	516	In stock (shipped next day)
96 T/48 S	948	In stock (shipped next day)

Warnings and Intended Use

This product is for research use only and is not intended for human or clinical diagnostic use. The use of this product may be subject to specific patents or licensing restrictions. Users are responsible for ensuring compliance with relevant laws and regulations. The company assumes no liability for any patent infringement or other violations that may arise from the use of this product.

Background Information

Pyroline-5-carboxylate synthase (P5CS) is a rate-limiting enzyme in the plant proline synthesis pathway. Under stress conditions (such as drought, salinity, low temperature, etc.), its upregulation significantly increases proline accumulation, enhancing plant stress resistance. This kit provides an effective tool for research on plant stress physiology.

Also Known As: Pyroline-5-Carboxylate Synthase (P5CS) | P5CS Activity Assay Kit

Assay Target: Pyroline-5-Carboxylate Synthase (P5CS) Activity

Note: The above information may be subject to change due to product optimization and upgrades. For specific details, please consult with Yakeyin Company.