S-NR试剂盒，用于样本中土壤硝酸还原酶活性检测，S-NR

Micro Soil Nitrate Reductase (S-NR) Activity Assay Kit

产品概述

本试剂盒（货号：KTB4010-E）是一种用于测定土壤样本中硝酸还原酶（S-NR）活性的比色法检测工具。该试剂盒通过测量酶促反应生成的红色偶氮化合物，在540 nm波长下进行吸光度测定，从而准确评估土壤中硝酸还原酶的活性水平。

应用领域

主要应用于土壤微生物代谢研究、环境科学中的水土检测以及农业生态系统的氮循环分析。

检测原理

土壤硝酸还原酶（S-NR）催化硝酸盐还原为亚硝酸盐（NO₃⁻ + NADH + H⁺ → NO₂⁻ + NAD⁺ + H₂O）。生成的亚硝酸盐在酸性条件下与对–氨`基`苯磺酸及α-萘`胺反应，形成红色偶氮化合物。该化合物在540 nm处具有最大吸收峰，可通过分光光度法进行定量分析。

试剂盒组成

• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• ReagentⅣ
• Standard（标准品）

产品特点

• 专为土壤样本中S-NR活性检测设计
• 提供标准品，便于结果计算与定量分析
• 包含详尽的样本制备与结果计算指南

规格与价格

保存与运输

建议收到货后避光保存于-20°C，有效期为12个月。运输条件为冰袋（蓝冰）运输，以确保试剂稳定性。

注意事项

操作时请穿戴合适的防护设备。本品仅供研究使用，不适用于人类或临床诊断。

警告

本产品仅用于科研目的。使用前请确保符合相关法律法规，并注意可能涉及的专利许可问题。对于因使用本产品而导致的任何专利侵权或违规行为，本公司不承担法律责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Soil Nitrate Reductase (S-NR) Activity Assay Kit

Product Overview

This assay kit (Cat. No.: KTB4010-E) is a colorimetric tool designed for the determination of nitrate reductase (S-NR) activity in soil samples. The kit accurately assesses soil nitrate reductase activity levels by measuring the absorbance at 540 nm of the red azo compound generated through enzymatic reaction.

Application Fields

Primarily applied in soil microbial metabolism research, water and soil testing in environmental science, and nitrogen cycle analysis in agricultural ecosystems.

Detection Principle

Soil nitrate reductase (S-NR) catalyzes the reduction of nitrate to nitrite (NO₃⁻ + NADH + H⁺ → NO₂⁻ + NAD⁺ + H₂O). The generated nitrite reacts with sulfanilic acid and α-naphthylamine under acidic conditions to form a red azo compound. This compound exhibits maximum absorbance at 540 nm, enabling quantitative analysis via spectrophotometry.

Kit Components

• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• ReagentⅣ
• Standard

Product Features

• Specifically designed for S-NR activity detection in soil samples
• Includes standard for convenient result calculation and quantitative analysis
• Comprehensive guidelines for sample preparation and result calculation

Specifications and Pricing

Storage and Shipping

Upon receipt, it is recommended to store protected from light at -20°C; the product is stable for 12 months. Shipping is conducted under ice pack (blue ice) conditions to ensure reagent stability.

Precautions

Appropriate protective equipment should be worn during operation. This product is for research use only and is not intended for human or clinical diagnostic use.

Warning

This product is for scientific research purposes only. Ensure compliance with relevant laws and regulations before use, and be aware of potential patent licensing issues. The company assumes no legal liability for any patent infringement or violations resulting from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Acrogen Co.