Glycerol试剂盒，用于样本中甘油含量检测，Glycer

CheKine™ 微量甘油含量检测试剂盒（Micro Glycerol Content Assay Kit）

货号：KTB2201-E

产品概述

CheKine™ 微量甘油含量检测试剂盒（Micro Glycerol Content Assay Kit）是一款基于比色法原理的高灵敏度检测工具，专为定量分析动植物组织、细胞、血清（浆）及其他液体样本中的甘油含量而设计。甘油作为甘油三酯代谢的关键中间产物，其含量变化可直接反映脂质代谢状态，是能量代谢研究中的重要指标。

检测原理

本试剂盒通过酶促反应链实现甘油定量：甘油在ATP存在下经甘油激酶催化生成3-磷酸甘油，随后在甘油磷酸氧化酶作用下氧化产生过氧化氢；过氧化氢在过氧化物酶催化下与生色底物反应生成苯`醌亚胺，在505 nm波长处具有特征吸收峰，通过测定吸光度值即可精确计算样本中甘油含量。

核心特点

• 广谱样本兼容性：经验证适用于动植物组织、细胞裂解液、血清（浆）及多种生物液体
• 操作流程简化：优化的反应体系无需复杂预处理，减少操作时间
• 高灵敏度检测：最低检测限达31.25 nmol/mL，精准捕捉低浓度代谢变化
• 宽线性范围：31.25-2,000 nmol/mL的检测范围满足绝大多数研究需求

试剂盒组分

• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard（甘油标准品）

技术参数

检测方法	比色法（505 nm）
样本类型	动植物组织、细胞、血清（浆）及其他液体
检测范围	31.25-2,000 nmol/mL
灵敏度	31.25 nmol/mL
保存条件	-20℃避光保存，有效期6个月
运输条件	蓝冰冰袋运输

使用建议

• 推荐使用新鲜样本，如需保存请置于-80℃（数周内稳定）
• 解冻样本需在4小时内完成（室温环境），避免反复冻融
• 实验前确保所有试剂及设备达到指定温度要求

规格与交付

规格	价格（元）	货期
48 T/48 S	439	现货（次日发货）
96 T/96 S	639	现货（次日发货）

注意事项

研究用途声明：本产品仅限科研使用，不适用于人类或临床诊断。使用者应自行确保应用场景不违反相关专利或许可协议，制造商不对专利侵权或违规使用承担责任。

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别名：甘油含量、甘油
检测指标：甘油（Glycerol）
分子：甘油（C₃H₈O₃）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Glycerol Content Assay Kit

Catalog Number: KTB2201-E

Product Overview

The CheKine™ Micro Glycerol Content Assay Kit is a highly sensitive colorimetric-based tool designed for the quantitative analysis of glycerol content in animal and plant tissues, cells, serum (plasma), and other liquid samples. Glycerol, as a key intermediate in triglyceride metabolism, exhibits concentration changes that directly reflect lipid metabolic status, making it an important indicator in energy metabolism research.

Assay Principle

This kit quantifies glycerol through an enzymatic reaction cascade: glycerol is phosphorylated by glycerol kinase in the presence of ATP to produce glycerol-3-phosphate, which is then oxidized by glycerol phosphate oxidase to generate hydrogen peroxide. The hydrogen peroxide, under the catalysis of peroxidase, reacts with a chromogenic substrate to form quinoneimine, which exhibits a characteristic absorption peak at 505 nm. The glycerol content in the sample can be precisely calculated by measuring the absorbance.

Key Features

• Broad Sample Compatibility: Validated for use with animal and plant tissues, cell lysates, serum (plasma), and various biological fluids
• Streamlined Workflow: Optimized reaction system requires no complex pre-treatment, reducing operation time
• High Sensitivity: Minimum detection limit of 31.25 nmol/mL, accurately capturing low-concentration metabolic changes
• Wide Linear Range: Detection range of 31.25–2,000 nmol/mL meets the needs of most research applications

Kit Components

• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard (Glycerol Standard)

Technical Parameters

Assay Method	Colorimetric (505 nm)
Sample Types	Animal/plant tissues, cells, serum (plasma), and other liquids
Detection Range	31.25–2,000 nmol/mL
Sensitivity	31.25 nmol/mL
Storage Conditions	Store protected from light at -20°C; valid for 6 months
Shipping Conditions	Shipped on blue ice packs

Usage Recommendations

• Fresh samples are recommended; store at -80°C if necessary (stable for several weeks)
• Thawed samples should be used within 4 hours (room temperature), avoid repeated freeze-thaw cycles
• Ensure all reagents and equipment reach the specified temperature requirements before the experiment

Specifications & Delivery

Specification	Price (CNY)	Lead Time
48 T / 48 S	439	In stock (shipped next day)
96 T / 96 S	639	In stock (shipped next day)

Precautions

Research Use Statement: This product is intended for research purposes only and is not intended for human or clinical diagnostic use. Users are responsible for ensuring that their application does not infringe any relevant patents or licensing agreements. The manufacturer assumes no liability for patent infringement or misuse.

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Also Known As: Glycerol Content, Glycerol
Assay Target: Glycerol
Molecule: Glycerol (C₃H₈O₃)

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Acadechem Company.