PDC试剂盒，用于样本中丙`酮酸脱羧酶检测，PDC Assa

CheKine™ 丙`酮酸脱羧酶（PDC）活性检测试剂盒（比色法）

货号：KTB1125-E

产品概述

CheKine™ Micro Pyruvate Decarboxylase (PDC) Assay Kit 是一款高特异性的比色法检测试剂盒，用于定量测定多种生物样本（包括植物组织、动物组织、细胞、细菌及血清/浆）中的丙`酮酸脱羧酶（PDC）活性。该试剂盒为三羧酸循环代谢研究提供关键工具，适用于基础科研与药物研发中的细胞代谢分析。

检测原理

本试剂盒基于丙`酮酸脱羧酶催化丙`酮酸脱羧生成乙醛的酶促反应，通过优化后的比色法检测体系，精确测量酶活性变化，确保实验数据的可靠性与重复性。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V

特点与优势

• 广泛样本兼容性：适用于植物/动物组织、细胞、细菌、血清（浆）等多种样本类型。
• 操作友好：提供详尽的样本制备指南与活性计算公式，降低操作门槛。
• 灵活调整：支持根据反应速率调整检测时间，优化实验灵敏度。

注意事项

• Extraction Buffer 内含不溶物，使用前需充分摇匀。
• 若1分钟内吸光度变化较小，可延长反应时间并相应调整计算公式。

保存与运输

保存条件：4℃避光保存，各组分具体存储条件请参见说明书。有效期自发货之日起6个月。

运输方式：冰袋（蓝冰）运输，确保试剂稳定性。

应用场景

本产品适用于乙醇发酵机制研究、细胞能量代谢分析、微生物生理功能探索等科研领域，尤其适合酵母及相关模型系统中PDC活性的精准检测。

规格与价格

规格	价格（元）	货期
48 T/48 S	848	现货（次日发货）
96 T/96 S	1326	现货（次日发货）

警告：本品仅供科研使用，不适用于临床诊断或人体应用。用户须自行确保使用方式不违反相关专利或许可协议。

别名：α-羧化酶, oxynitrilase, pyruvate decarboxylase

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Pyruvate Decarboxylase (PDC) Activity Assay Kit (Colorimetric Method)

Catalog Number:KTB1125-E

Overview

The CheKine™ Micro Pyruvate Decarboxylase (PDC) Assay Kit is a highly specific colorimetric assay kit designed for the quantitative determination of pyruvate decarboxylase (PDC) activity in various biological samples, including plant tissues, animal tissues, cells, bacteria, and serum/plasma. This kit provides a critical tool for research on the tricarboxylic acid (TCA) cycle metabolism and is suitable for cell metabolism analysis in basic scientific research and drug development.

Principle

This kit is based on the enzymatic reaction in which pyruvate decarboxylase catalyzes the decarboxylation of pyruvate to produce acetaldehyde. Through an optimized colorimetric detection system, it accurately measures changes in enzyme activity, ensuring reliable and reproducible experimental data.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V

Features and Advantages

• Broad Sample Compatibility: Suitable for various sample types, including plant/animal tissues, cells, bacteria, and serum/plasma.
• User-Friendly Operation: Provides detailed sample preparation guidelines and activity calculation formulas to reduce operational barriers.
• Flexible Adjustment: Supports adjustment of detection time based on reaction rate to optimize experimental sensitivity.

Precautions

• The Extraction Buffer contains insoluble substances; shake well before use.
• If the change in absorbance within 1 minute is minimal, the reaction time can be extended, and the calculation formula should be adjusted accordingly.

Storage and Shipping

Storage Conditions: Store protected from light at 4°C. Refer to the manual for specific storage conditions of each component. The validity period is 6 months from the date of shipment.

Shipping Method: Shipped with ice packs (blue ice) to ensure reagent stability.

Applications

This product is suitable for research areas such as ethanol fermentation mechanisms, cellular energy metabolism analysis, and exploration of microbial physiological functions. It is particularly ideal for the accurate detection of PDC activity in yeast and related model systems.

Specifications and Pricing

Specification	Price (¥)	Availability
48 T/48 S	848	In stock (ships next day)
96 T/96 S	1326	In stock (ships next day)

Warning: This product is for research use only and is not intended for clinical diagnosis or human use. Users must ensure that their use does not violate any relevant patents or licensing agreements.

Aliases: α-carboxylase, oxynitrilase, pyruvate decarboxylase

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Applygen Technologies Inc.