离子结合型果胶试剂盒，用于样本中ISP含量检测，微量法，IS

CheKine™ 离子结合型果胶（ISP）含量检测试剂盒（微量法）

货号：KTB3016-S

产品类型：细胞代谢检测试剂盒

样本类型：植物组织

产品概述

CheKine™ 离子结合型果胶（ISP）含量检测试剂盒（微量法）是一款专为植物组织样本中离子结合型果胶（ISP）含量精确测定而设计的核心产品。本试剂盒采用微量法操作，流程简便，适用于细胞代谢研究及细胞分析应用领域，可帮助研究人员高效评估植物细胞壁中果胶成分的含量与状态。

技术原理

本试剂盒基于咔`唑比色法原理，利用带有螯合剂的酸溶液提取样本中的离子结合型果胶（ISP），并在酸性条件下使果胶水解为半乳糖醛酸。随后，水解产物与咔`唑试剂发生缩合反应，生成在530 nm波长处具有特征吸收峰的复合物，通过测定吸光度值即可准确计算ISP含量。

试剂盒组分

• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

特点与优势

• 验证的样本类型齐全，涵盖多种植物组织
• 操作流程简便，减少实验复杂度
• 提供标准品，便于定量与结果校准

适用样本与保存建议

推荐使用新鲜植物组织样本进行检测。如不立即实验，样本可在-80℃条件下保存数周。测定前需注意控制解冻温度与时间，室温解冻应在4小时内完成。

保存与运输

试剂盒需在4℃避光条件下保存，有效期为6个月。运输过程中采用冰袋（蓝冰）保障低温环境，确保试剂稳定性。

可选规格与价格

• 48 T/48 S：¥478（现货，次日发货）
• 96 T/96 S：¥898（现货，次日发货）

注意事项

实验前请确保所有组分及设备处于适宜温度。本产品仅供科研研究使用，不适用于人类或临床诊断。使用前请查阅相关专利或许可条款，确保符合法规要求。

警告：本文所列产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Ion-Bound Pectin (ISP) Content Assay Kit (Micro-scale Method)

Cat. No.:KTB3016-S

Product Type:Cell Metabolism Assay Kit

Sample Type:Plant Tissue

Product Overview

The CheKine™ Ion-Bound Pectin (ISP) Content Assay Kit (Micro-scale Method) is a core product specifically designed for the accurate determination of ion-bound pectin (ISP) content in plant tissue samples. This kit utilizes a micro-scale method with a simple procedure, making it suitable for cell metabolism research and cellular analysis applications. It enables researchers to efficiently evaluate the content and status of pectin components in plant cell walls.

Technical Principle

This kit is based on the carbazole colorimetric principle. It uses an acid solution containing a chelating agent to extract ion-bound pectin (ISP) from the sample, hydrolyzing the pectin into galacturonic acid under acidic conditions. Subsequently, the hydrolysis products undergo a condensation reaction with the carbazole reagent to form a complex with a characteristic absorption peak at 530 nm. The ISP content can be accurately calculated by measuring the absorbance value.

Kit Components

• Reagent I
• Reagent II
• Reagent III
• Standard

Features and Advantages

• Validated for a wide range of sample types, including various plant tissues
• Simple operational procedure reduces experimental complexity
• Includes standard for convenient quantification and result calibration

Applicable Samples and Storage Recommendations

Fresh plant tissue samples are recommended for detection. If not used immediately, samples can be stored at -80°C for several weeks. Prior to assay, control thawing temperature and time carefully; thawing at room temperature should be completed within 4 hours.

Storage and Shipping

The kit should be stored protected from light at 4°C and is stable for 6 months. During shipping, ice packs (blue ice) are used to maintain a low-temperature environment, ensuring reagent stability.

Available Sizes and Pricing

• 48 T/48 S: ¥478 (In stock, ships next day)
• 96 T/96 S: ¥898 (In stock, ships next day)

Precautions

Before the experiment, ensure all components and equipment are at appropriate temperatures. This product is for scientific research use only and is not intended for human or clinical diagnostic use. Please review relevant patent or licensing terms prior to use to ensure compliance with regulatory requirements.

Warning: The products listed herein are for research use only and are not intended for human or clinical diagnosis. The applications recommended for our products are not a suggestion to use our products to infringe any patents or licenses. We assume no liability for patent infringement or other violations that may occur with the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult YaKeyin Company.