谷丙转氨酶试剂盒，用于样本中ALT/GPT活性检测，微量法，

谷丙转氨酶(ALT/GPT)活性检测试剂盒 (微量法)

货号：KTB1410-S

规格：96 T/48 S

价格：¥198

库存状态：现货，支持当天发货

产品概述

本试剂盒是一款基于比色法原理，专业用于检测各类生物样本中谷丙转氨酶（ALT/GPT）活性的核心产品。其设计针对细胞代谢研究领域，适用于多种样本类型，包括动物组织、植物组织、血清、细胞以及细菌等，为氨基酸代谢途径的研究提供关键检测工具。

产品特点与优势

• 广泛的应用范围：可精确测定多种生物样本（动物组织、植物组织、血清、细胞、细菌等）中的ALT/GPT酶活性。
• 全面的实验支持：试剂盒提供了详尽的样本制备指南与结果计算方法，并包含标准品与标准曲线作参考，助力实验的标准化与重复性。
• 便捷的检测流程：采用成熟的比色法检测方法，操作流程清晰，便于在实验室环境中高效开展。

检测原理

谷丙转氨酶（ALT/GPT）催化丙氨酸和α-酮戊二酸之间的转氨基反应，生成丙`酮酸和谷氨酸。随后，丙`酮酸与特定的显色试剂反应生成有色产物，其在505 nm波长处的吸光度值与丙`酮酸含量成正比，从而可间接精确计算出ALT/GTP的活性水平。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Standard

背景信息

谷丙转氨酶（ALT/GPT）是氨基酸代谢过程中的关键酶，广泛存在于动物、植物、微生物及培养细胞中。在临床与基础研究中，血清中的ALT活性是评估肝脏功能与肝细胞损伤程度的重要生化指标。同时，其在心肌细胞中亦有高含量，常作为心肌梗塞和心肌炎研究的辅助检查指标。

注意事项

• 请勿混合使用不同批次号或不同厂家的试剂组件，以保证检测结果的准确性。
• 样本处理及实验操作过程需在冰上进行，以维持酶活性。样本匀浆液应避免反复冻融，且酶活测定须在当日完成。
• 实验前，请确保所有试剂组件及所需设备均已恢复或处于合适的反应温度。

保存与运输

保存条件：请收到产品后参照说明书建议的条件保存各组件。产品自发货之日起，在推荐的保存条件下，有效期为6个月。

运输条件：全程采用蓝冰（冰袋）低温运输，以确保试剂稳定性。

重要声明

本产品仅限于科学研究用途，不适用于人类或临床诊断。我们所推荐的应用领域并不代表建议用户利用本产品去违反任何专利或许可证规定。对于因使用本产品而可能导致的专利侵权或其他违规行为，本公司不承担任何责任。

别名检索： 谷丙转氨酶, 谷丙转氨酶（ALT/GPT）活性检测, 谷丙转氨酶（ALT/GPT）活性检测试剂盒, ALT/GPT, Alanine Aminotransferase, ALT

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Micro Method)

Catalog Number: KTB1410-S

Specification: 96 T/48 S

Price: ¥198

Stock Status: In stock, supports same-day shipping

Product Overview

This kit is a core product based on the colorimetric principle, professionally designed for detecting alanine aminotransferase (ALT/GPT) activity in various biological samples. Its design is tailored for the field of cell metabolism research and is suitable for multiple sample types, including animal tissues, plant tissues, serum, cells, and bacteria, providing a key detection tool for studying amino acid metabolic pathways.

Features and Advantages

• Broad Application Range: Precisely measures ALT/GPT enzyme activity in various biological samples (animal tissues, plant tissues, serum, cells, bacteria, etc.).
• Comprehensive Experimental Support: The kit provides detailed sample preparation guidelines and result calculation methods, and includes standards and standard curves for reference, facilitating experimental standardization and reproducibility.
• Convenient Detection Process: Utilizes a well-established colorimetric detection method with a clear operational procedure, enabling efficient execution in a laboratory environment.

Assay Principle

Alanine aminotransferase (ALT/GPT) catalyzes the transamination reaction between alanine and α-ketoglutarate, producing pyruvate and glutamate. Subsequently, pyruvate reacts with a specific chromogenic reagent to generate a colored product, whose absorbance at 505 nm is proportional to the pyruvate content, thereby allowing for the indirect and precise calculation of ALT/GPT activity levels.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Standard

Background Information

Alanine aminotransferase (ALT/GPT) is a key enzyme in the amino acid metabolism process, widely present in animals, plants, microorganisms, and cultured cells. In clinical and basic research, serum ALT activity is an important biochemical indicator for assessing liver function and the extent of hepatocyte damage. It is also present in high concentrations in myocardial cells and is often used as an auxiliary examination indicator for myocardial infarction and myocarditis research.

Precautions

• Do not mix reagents from different lot numbers or different manufacturers to ensure the accuracy of the detection results.
• Sample processing and experimental operations should be performed on ice to maintain enzyme activity. Sample homogenates should avoid repeated freeze-thaw cycles, and enzyme activity assays must be completed on the same day.
• Before the experiment, ensure all reagent components and required equipment have been returned to or are at the appropriate reaction temperature.

Storage and Shipping

Storage Conditions: Upon receipt, please store all components according to the conditions recommended in the manual. The product is valid for 6 months from the date of shipment when stored under the recommended conditions.

Shipping Conditions: Shipped at low temperatures using blue ice (ice packs) throughout to ensure reagent stability.

Important Statement

This product is intended for research use only and is not suitable for human or clinical diagnostic use. The application areas we recommend do not suggest that users should utilize this product to violate any patent or license regulations. Our company assumes no liability for any patent infringement or other violations that may result from the use of this product.

Alias Search: 谷丙转氨酶, 谷丙转氨酶（ALT/GPT）活性检测, 谷丙转氨酶（ALT/GPT）活性检测试剂盒, ALT/GPT, Alanine Aminotransferase, ALT

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakin Company.