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Gamma-Adaptin Rabbit Polyclona

l Antibody抗体,orb10106,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb10106
  • 英国
  • 2025年12月03日
  • ICC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Gamma-Adaptin Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Gamma-Adaptin Rabbit Polyclonal Antibody

    • 靶点

      AP1G1

    • 浓度

      1mg/ml

    • 应用范围

      ICC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb10106

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human Gamma-Adaptin (721-822/822aa)

    • 规格

      50 ul

    产品描述:Gamma-Adaptin Rabbit Polyclonal Antibody

    别名:Adapter-related protein complex 1 subunit gamma-1; Adaptor protein complex AP 1 gamma 1 subunit; Adaptor protein complex AP 1 subunit gamma 1; Adaptor protein complex AP-1 subunit gamma-1; Adaptor related protein complex 1 gamma 1 subunit; adaptor-related protein complex 1 gamma 1 subunit; ADTG; ADTG; AP 1 complex subunit gamma 1; AP-1 complex subunit gamma-1; AP1G1; AP1G1_HUMAN; CLAPG1; CLAPG1; Clathrin assembly protein complex 1 gamma 1 large chain; Clathrin assembly protein complex 1 gamma large chain; Clathrin assembly protein complex 1 gamma-1 large chain; Clathrin associated/assembly/adaptor protein large gamma 1; clathrin-associated/assembly/adaptor protein large gamma 1; gamma1 adaptin; gamma1-adaptin; Golgi adaptor HA1/AP1 adaptin gamma subunit; Golgi adaptor HA1/AP1 adaptin subunit gamma 1; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; MGC18255.

    免疫原:KLH conjugated synthetic peptide derived from human Gamma-Adaptin (721-822/822aa)

    预测反应性:Bovine, Canine, Gallus, Porcine, Rabbit

    分子量:91 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:O43747

    Note:For research use only.

    产品细节图片1
    Cell culture (MCF7), Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (orb10106) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining.

    产品细节图片2
    Cell culture (SW480), Fixative-fixed, Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (orb10106) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (orb10106) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Sample: 293T (Human) Cell Lysate at 30 ug, Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Gamma-Adaptin (orb10106) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 91 kD, Observed band size: 91 kD.

    产品细节图片5
    Tissue/cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (orb10106) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

    产品细节图片6
    Tissue/cell: Rat brian, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Gamma-Adaptin, Unconjugated (orb10106) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

    产品细节图片7
    Tissue/cell: rat lung carcinoma, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (orb10106) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
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