γ-谷氨酰半胱氨酸连接酶活性检测试剂盒，用于对样本中GCL活

γ-谷氨酰半胱氨酸连接酶（GCL）活性检测试剂盒

货号：KTB1680-C

英文名称：Micro γ-Glutamyl Cysteine Ligase (GCL) Activity Assay Kit

产品类型：细胞代谢检测试剂盒

产品分类：核心产品

产品概述

本试剂盒为比色法检测试剂盒，用于精准、可靠地定量检测多种生物样本（包括细胞、细菌、真菌、血清及动植物组织）中的γ-谷氨酰半胱氨酸连接酶（GCL）活性。GCL是细胞内合成谷胱甘肽（GSH）的关键限速酶，其活性高低直接影响细胞内GSH含量及氧化还原平衡状态（GSH/GSSG比值）。

检测原理

试剂盒基于经典的生化比色反应原理，通过检测与GCL催化反应直接相关的产物生成速率，从而准确计算出样本中GCL的酶活性水平。实验提供了标准品与标准曲线作参考，确保结果的准确性与可比性。

试剂盒组分

• Extraction Buffer
• Reagent I A
• Reagent I B
• Reagent II
• Reagent III
• Reagent IV
• Standard

特点与优势

• 广泛样本兼容性：适用于细胞、细菌、真菌、血清及动植物组织等多种样本类型。
• 操作便捷：提供了详尽的样本制备指南与结果计算公式，简化实验流程。
• 结果可靠：内含标准品，可制作标准曲线，为定量分析提供坚实依据。

规格与定价

规格	价格 (元)	货期
48 T/48 S	378	现货（次日发货）
96 T/96 S	598	现货（次日发货）

注意事项

• 严禁混合使用不同批次号或不同厂家的试剂组分。
• 样本处理及实验操作需全程在冰上进行，以确保酶活性不受影响。检测应在当日完成。组织匀浆液应避免反复冻融。
• 实验过程中请勤换吸头，防止各组分间出现交叉污染。
• 试剂混合或复溶时，请轻柔操作，避免产生气泡。
• 实验前，请确认所有试剂及所需设备均已恢复至合适的温度。

保存与运输

保存条件：收到产品后，请避光保存于-20℃。请详细参阅说明书了解各组分具体存储条件。在推荐保存条件下，自发货之日起，产品有效期为6个月。

运输条件：全程采用冰袋（蓝冰）低温运输，以确保试剂稳定性。

警告

本产品仅限于科研用途，不适用于人类或临床诊断。产品推荐应用不构成任何专利或许可证违规使用的建议。对于使用本产品可能导致的任何专利侵权或其他违规行为，本公司不承担任何责任。

背景信息

γ-谷氨酰半胱氨酸连接酶（GCL）是细胞内合成谷胱甘肽（GSH）的限速酶，其自身活性受到GSH的反馈抑制调控。GCL的基因表达受氧化剂、抗氧化剂、生长因子及炎症因子等多种因素的复杂调节。因此，精确检测GCL活性对于研究细胞抗氧化应激、代谢调控及相关生理病理过程至关重要。

别名：γ-谷氨酰半胱氨酸连接酶（GCL）, GCL, 谷氨酰半胱氨酸连接酶

检测指标：GCL

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

γ-Glutamylcysteine Ligase (GCL) Activity Assay Kit

Catalog Number: KTB1680-C

English Name: Micro γ-Glutamyl Cysteine Ligase (GCL) Activity Assay Kit

Product Type: Cell Metabolism Assay Kit

Product Classification: Core Product

Product Overview

This kit is a colorimetric assay kit designed for the accurate and reliable quantitative detection of γ-glutamylcysteine ligase (GCL) activity in a variety of biological samples, including cells, bacteria, fungi, serum, and animal/plant tissues. GCL is the key rate-limiting enzyme in the intracellular synthesis of glutathione (GSH), and its activity directly influences intracellular GSH levels and the redox balance state (GSH/GSSG ratio).

Assay Principle

The kit is based on the classic biochemical colorimetric reaction principle. It accurately calculates the enzymatic activity level of GCL in samples by detecting the rate of production directly related to the GCL-catalyzed reaction. The experiment includes a standard and a standard curve for reference, ensuring the accuracy and comparability of results.

Kit Components

• Extraction Buffer
• Reagent I A
• Reagent I B
• Reagent II
• Reagent III
• Reagent IV
• Standard

Features and Advantages

• Broad Sample Compatibility: Suitable for various sample types including cells, bacteria, fungi, serum, and animal/plant tissues.
• Convenient Operation: Provides detailed sample preparation guidelines and result calculation formulas to simplify the experimental procedure.
• Reliable Results: Includes a standard for generating a standard curve, providing a solid basis for quantitative analysis.

Specifications and Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	378	In Stock (Ships Next Day)
96 T/96 S	598	In Stock (Ships Next Day)

Precautions

• Do not mix components from different lot numbers or different manufacturers.
• Sample handling and experimental operations must be performed on ice throughout to ensure enzyme activity is not compromised. Assays should be completed on the same day. Avoid repeated freeze-thaw cycles of tissue homogenates.
• Change pipette tips frequently during the experiment to prevent cross-contamination between components.
• Mix or reconstitute reagents gently to avoid generating bubbles.
• Before starting the experiment, ensure all reagents and required equipment have equilibrated to the appropriate temperature.

Storage and Shipping

Storage Conditions: Upon receipt, store the product protected from light at -20°C. Please refer to the manual for specific storage conditions of individual components. Under recommended storage conditions, the product is stable for 6 months from the date of shipment.

Shipping Conditions: The product is shipped with ice packs (blue ice) to ensure reagent stability during transit.

Warning

This product is intended for research use only and is not intended for human or clinical diagnostic use. Recommended applications do not constitute a suggestion to infringe any patents or licenses. The company assumes no liability for any patent infringement or other violations that may result from the use of this product.

Background Information

γ-Glutamylcysteine ligase (GCL) is the rate-limiting enzyme in the intracellular synthesis of glutathione (GSH), and its activity is subject to feedback inhibition by GSH. The gene expression of GCL is complexly regulated by various factors, including oxidants, antioxidants, growth factors, and inflammatory cytokines. Therefore, precise detection of GCL activity is crucial for studying cellular antioxidant stress, metabolic regulation, and related physiological and pathological processes.

Alias: γ-Glutamylcysteine Ligase (GCL), GCL, Glutamate-Cysteine Ligase

Detection Target: GCL

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yaken Company.