单宁酶活性检测试剂盒，用于对样本中Tannase活性检测，T

单宁酶（TAN）活性检测试剂盒

产品货号：KTB1542-C

产品类型：细胞代谢检测试剂盒（比色法）

英文名称：Micro Tannase (TAN) Activity Assay Kit

产品概述

本试剂盒是一种高灵敏度的体外比色法检测工具，专为定量检测多种生物样本（包括植物组织、真菌和细菌）中的单宁酶（Tannase, TAN）活性而设计。适用于氧化应激等相关细胞代谢研究领域，提供可靠的检测性能与标准曲线参考。

检测原理

试剂盒基于单宁酶水解酯键和缩酚羧键的特性，通过比色法在特定波长下（需使用96孔UV微孔板）检测酶促反应产物，从而准确计算酶活性水平。

组分内容

• Extraction Buffer
• Substrate
• Standard

特点与优势

• 广泛样本兼容性：适用于植物、真菌、细菌等多种来源样本；
• 提供详细操作指南：含样本制备、反应步骤及结果计算方法的明确说明；
• 包含标准品与标准曲线：便于定量分析及结果校准。

技术参数

检测范围：0.3125 - 20 μmoL/mL

检测方法：比色法

样本类型：植物组织、真菌、细菌等提取液

注意事项

• 需使用96孔UV微孔板进行检测；
• 禁止混用不同批次或厂家的试剂组分；
• 样品处理需在冰上操作，并于当日完成测定，避免反复冻融；
• 实验前请确保所有试剂及设备处于适宜温度。

保存与运输

保存条件：避光保存于4℃，有效期6个月。各组分具体保存条件请参见说明书。

运输方式：冰袋（蓝冰）运输。

规格与价格

规格	价格（元）	货期
48 T/48 S	626	现货（次日发货）
96 T/96 S	898	现货（次日发货）

背景信息

单宁酶（Tannase，EC 3.1.1.20），亦称鞣酸酶或单宁酰基水解酶，广泛存在于富含单宁的植物及微生物中。该酶可催化水解酯键与缩酚羧键，生成没食子酸与葡萄糖，在食品工业（如啤酒澄清、柿子脱涩、速溶茶制备）及微生物代谢研究中具有重要应用。

别名：单宁酶, 丹宁酶, Tannase

搜索关键词：单宁酶, 丹宁酶, Tannase, TAN活性检测, 细胞代谢试剂盒

警告：本产品仅用于科学研究，不适用于人类或临床诊断。使用者应确保不侵犯任何专利或许可证，制造商不对因使用本产品导致的专利侵权或其他违规行为负责。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Tannase (TAN) Activity Assay Kit

Catalog Number:KTB1542-C

Product Type:Cell Metabolism Assay Kit (Colorimetric Method)

English Name:Micro Tannase (TAN) Activity Assay Kit

Product Overview

This kit is a highly sensitive in vitro colorimetric assay tool specifically designed for the quantitative detection of Tannase (TAN) activity in various biological samples, including plant tissues, fungi, and bacteria. It is suitable for research areas related to oxidative stress and other cellular metabolism studies, providing reliable assay performance and a standard curve for reference.

Assay Principle

The kit is based on the characteristic of tannase hydrolyzing ester bonds and depside bonds. The enzymatic reaction products are detected colorimetrically at a specific wavelength (requires the use of a 96-well UV microplate) to accurately calculate the enzyme activity level.

Kit Components

• Extraction Buffer
• Substrate
• Standard

Features & Advantages

• Broad Sample Compatibility: Suitable for samples from various sources such as plants, fungi, and bacteria;
• Detailed Protocol Provided: Includes clear instructions for sample preparation, reaction procedures, and calculation methods;
• Includes Standard and Standard Curve: Facilitates quantitative analysis and result calibration.

Technical Parameters

Detection Range:0.3125 - 20 μmoL/mL

Assay Method:Colorimetric Method

Sample Type:Extracts from plant tissues, fungi, bacteria, etc.

Precautions

• Requires the use of a 96-well UV microplate for detection;
• Do not mix components from different batches or manufacturers;
• Sample handling should be performed on ice, and assays completed on the same day; avoid repeated freeze-thaw cycles;
• Ensure all reagents and equipment are at appropriate temperatures prior to the experiment.

Storage & Shipping

Storage Conditions:Store protected from light at 4°C, stable for 6 months. Refer to the manual for specific storage conditions of each component.

Shipping Method:Shipped with ice packs (blue ice).

Specifications & Pricing

Specification	Price (¥)	Availability
48 T/48 S	626	In Stock (Ships Next Day)
96 T/96 S	898	In Stock (Ships Next Day)

Background Information

Tannase (Tannase, EC 3.1.1.20), also known as tannin acyl hydrolase, is widely found in tannin-rich plants and microorganisms. This enzyme catalyzes the hydrolysis of ester bonds and depside bonds, producing gallic acid and glucose. It has significant applications in the food industry (e.g., beer clarification, persimmon de-astringency, instant tea preparation) and in microbial metabolism research.

Also Known As:Tannase, Tannin Acyl Hydrolase

Search Keywords:Tannase, TAN Activity Assay, Cell Metabolism Assay Kit

Warning:This product is for research use only. Not intended for human or diagnostic use. The user should ensure that no patents or licenses are infringed; the manufacturer is not responsible for patent infringement or other violations resulting from the use of this product.

Note: The above information is subject to change due to product optimization and upgrades. Please consult Yakeyin Company for specific details.