土壤酸性木聚糖酶试剂盒，用于样本中S-ACX活性检测，微量法

土壤酸性木聚糖酶（S-ACX）活性检测试剂盒（微量法）

货号：KTB4056-S

产品分类：核心产品

产品中心：细胞分析

产品概述

CheKine™ 土壤酸性木聚糖酶（S-ACX）活性检测试剂盒（微量法）是一款专为土壤样本设计的检测工具，用于准确测定样本中酸性木聚糖酶的活性。本试剂盒采用微量法操作，适用于环境科学、农业研究及微生物代谢分析等领域。

背景信息

木聚糖酶（EC3.2.1.8），又称戊聚糖酶或半纤维素酶，主要由微生物分泌，能够催化水解木聚糖。酸性木聚糖酶（S-ACX）常见于耐酸真菌、细菌及部分霉菌，在酿造和饲料工业中具有重要应用价值，可有效降解原料细胞壁，降低物料粘度，并促进营养物质的释放与吸收。

检测原理

本试剂盒基于显色反应原理。在酸性条件下，S-ACX 能够将木聚糖降解为还原性寡糖和单糖。这些还原糖在沸水浴中与3,5-二硝基水杨酸发生反应，生成在540 nm波长处具有特征吸收峰的化合物。通过测定吸光度值的增加速率，可准确计算出S-ACX的酶活性。

试剂盒组分

• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent V
• Reagent VI
• Standard

特点与优势

• 已验证适用于多种土壤样本类型
• 操作流程简便，检测快速
• 提供标准品，便于结果计算与比对

样本类型

土壤样本

注意事项

• 推荐使用新鲜样本。如不立即进行实验，样本可在-80℃保存数周。
• 测定时需严格控制解冻温度与时间。室温解冻应在4小时内完成。
• 实验前请确保所有试剂及设备处于适宜温度。

保存与运输

保存条件：4℃避光保存，有效期6个月。

运输条件：冰袋（蓝冰）运输。

可选规格与价格

规格	价格（元）	货期
48 T/48 S	378	现货（次日发货）
96 T/96 S	689	现货（次日发货）

警告

本产品仅供研究使用，不适用于人类或临床诊断。使用本产品可能涉及专利或许可证限制，对此我们不承担任何责任。

别名：土壤酸性木聚糖酶（S-ACX）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Soil Acidic Xylanase (S-ACX) Activity Assay Kit (Micro Method)

Cat. No.:KTB4056-S

Product Classification:Core Products

Product Center:Cell Analysis

Product Overview

CheKine™ Soil Acidic Xylanase (S-ACX) Activity Assay Kit (Micro Method) is a detection tool specifically designed for soil samples to accurately determine the activity of acidic xylanase in samples. This kit employs a micro method operation and is suitable for environmental science, agricultural research, and microbial metabolism analysis.

Background

Xylanase (EC 3.2.1.8), also known as pentosanase or hemicellulase, is primarily secreted by microorganisms and catalyzes the hydrolysis of xylan. Acidic xylanase (S-ACX) is commonly found in acid-tolerant fungi, bacteria, and some molds. It holds significant application value in brewing and feed industries, effectively degrading raw material cell walls, reducing material viscosity, and promoting the release and absorption of nutrients.

Assay Principle

This kit is based on a colorimetric reaction principle. Under acidic conditions, S-ACX can degrade xylan into reducing oligosaccharides and monosaccharides. These reducing sugars react with 3,5-dinitrosalicylic acid in a boiling water bath to generate a compound with a characteristic absorption peak at 540 nm. By measuring the rate of increase in absorbance, the enzymatic activity of S-ACX can be accurately calculated.

Kit Components

• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent V
• Reagent VI
• Standard

Features & Advantages

• Validated for various soil sample types
• Simple procedure and rapid detection
• Includes standard for easy result calculation and comparison

Sample Type

Soil samples

Precautions

• Fresh samples are recommended. If not used immediately, samples can be stored at -80°C for several weeks.
• Strictly control thawing temperature and time during assay. Thawing at room temperature should be completed within 4 hours.
• Ensure all reagents and equipment are at appropriate temperatures before the experiment.

Storage & Shipping

Storage Conditions:Store protected from light at 4°C; valid for 6 months.

Shipping Conditions:Shipped with ice packs (blue ice).

Available Specifications & Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	378	In stock (ships next day)
96 T/96 S	689	In stock (ships next day)

Warning

This product is for research use only. Not intended for human or clinical diagnostic use. Use of this product may involve patent or license restrictions, for which we assume no liability.

Alias:Soil Acidic Xylanase (S-ACX)

Note: The above information may change due to product optimization and upgrades. For specific details, please consult Yakeyin Company.