土壤荧光素二乙酸酯水解酶试剂盒，用于样本中S-FDA活性检测

CheKine™ 土壤荧光素二乙酸酯水解酶（S-FDA）活性检测试剂盒（微量法）

货号：KTB4049-S

产品概述

CheKine™ 土壤荧光素二乙酸酯水解酶（S-FDA）活性检测试剂盒（微量法）是一款基于比色法原理，用于定量检测土壤样本中S-FDA水解酶活性的专业试剂盒。该试剂盒通过检测490 nm波长下荧光素的吸光度变化，精准反映土壤微生物活性及有机质转化能力，是评估土壤质量和生态系统健康的重要工具。

检测原理

荧光素二乙酸酯（FDA）作为一种无色底物，在土壤酶催化下发生水解及脱水反应，生成稳定的荧光素。该产物在490 nm处具有特征吸收峰，通过微量法测定吸光值变化，即可准确计算出S-FDA水解酶的活性水平。

组分清单

• Reagent I
• Reagent Ⅱ
• Standard

样本类型

本试剂盒适用于土壤样本的检测。推荐使用新鲜样本，若需保存，请于-80℃条件下存放，并在数周内完成实验。解冻过程需严格控制温度与时间，室温解冻应在4小时内完成。

特点与优势

• 经验证的样本类型齐全，适用性强
• 操作流程简便，检测结果稳定可靠

保存与运输

试剂盒需在4℃条件下避光保存，有效期6个月。运输过程中采用蓝冰（冰袋）保温，以确保试剂稳定性。

注意事项

• 实验前请确保所有组分及设备处于适宜温度
• 本产品仅限研究使用，不适用于人类或临床诊断

规格与价格

背景与应用

FDA水解酶活性是评价土壤微生物群落功能与有机质转化效率的关键生物学指标，广泛应用于环境科学研究、农业土壤质量监测及生态评估等领域。

别名：土壤荧光素二乙酸酯水解酶（S-FDA）

检测指标：土壤荧光素二乙酸酯水解酶（S-FDA）活性

注：本品仅提供研究用途，不承担因使用本品可能涉及的专利侵权或其他违规行为责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Soil Fluorescein Diacetate Hydrolase (S-FDA) Activity Assay Kit (Micro Method)

Catalog Number: KTB4049-S

Product Overview

The CheKine™ Soil Fluorescein Diacetate Hydrolase (S-FDA) Activity Assay Kit (Micro Method) is a professional kit based on the colorimetric principle for the quantitative detection of S-FDA hydrolase activity in soil samples. This kit accurately reflects soil microbial activity and organic matter transformation capacity by measuring changes in absorbance of fluorescein at 490 nm, serving as an essential tool for assessing soil quality and ecosystem health.

Assay Principle

Fluorescein diacetate (FDA), as a colorless substrate, undergoes hydrolysis and dehydration reactions catalyzed by soil enzymes to generate stable fluorescein. This product exhibits a characteristic absorption peak at 490 nm, and the activity level of S-FDA hydrolase can be accurately calculated by measuring the change in absorbance using the micro method.

Component List

• Reagent I
• Reagent Ⅱ
• Standard

Sample Type

This kit is suitable for the detection of soil samples. Fresh samples are recommended. If storage is necessary, samples should be kept at -80°C and experiments completed within a few weeks. The thawing process must strictly control temperature and time; thawing at room temperature should be completed within 4 hours.

Features and Advantages

• Comprehensive validated sample types with strong applicability
• Simple operation process with stable and reliable detection results

Storage and Transportation

The kit should be stored protected from light at 4°C and is valid for 6 months. During transportation, blue ice (ice packs) are used for thermal insulation to ensure reagent stability.

Precautions

• Before the experiment, ensure all components and equipment are at appropriate temperatures.
• This product is for research use only and is not intended for human or clinical diagnostic use.

Specifications and Pricing

Background and Application

FDA hydrolase activity is a key biological indicator for evaluating soil microbial community function and organic matter transformation efficiency, widely used in environmental science research, agricultural soil quality monitoring, and ecological assessment.

Alias: Soil Fluorescein Diacetate Hydrolase (S-FDA)

Detection Target: Soil Fluorescein Diacetate Hydrolase (S-FDA) Activity

Note: This product is provided for research purposes only. The company does not assume responsibility for any patent infringement or other violations that may arise from the use of this product.

Note: The above information may change due to product optimization and upgrades. For specific details, please consult Yakeyin Company.