活性氧试剂盒，用于样本中ROS含量检测，ROS Detect

活性氧（ROS）检测试剂盒（荧光法）

货号：KTB1913-S

产品类型：细胞代谢检测试剂盒

应用领域：细胞代谢研究、氧化应激分析、心血管疾病、神经退行性疾病、癌症机制研究等

产品概述

本试剂盒为基于荧光法的活性氧（Reactive Oxygen Species, ROS）检测工具，适用于活细胞或新鲜组织样本中ROS含量的定量分析。试剂盒采用高灵敏度荧光探针DHE（二氢乙锭），可在流式细胞仪或荧光显微镜下实现对ROS的高效、可靠检测。

主要组分

• DHE (1000×)
• ROS Positive Control (1000×)

样本类型

适用于活细胞及新鲜组织样本。

检测方法

荧光法（Fluorometric Assay），兼容流式细胞术与荧光显微镜成像分析。

特点与优势

• 灵敏度高，适用于低浓度ROS检测；
• 适用于多种样本类型，灵活性高；
• 阳性对照提供系统质控，结果更可靠。

注意事项

• 建议进行预实验以优化检测条件；
• 推荐使用BCA法对样本进行蛋白浓度均一化处理；
• 用户需自行建立标准曲线；
• 操作时请于通风橱中进行，佩戴手套等防护用具，避免直接接触试剂。

保存与运输

保存条件：避光，-20℃保存，有效期12个月。

运输条件：冰袋（蓝冰）运输，保障试剂稳定性。

警告

本品属生化试剂，具有一定刺激性与毒性，请严格遵守操作规范。

背景信息

活性氧（ROS）是细胞有氧代谢过程中的副产物，参与细胞信号转导与稳态维持。在氧化应激状态下，ROS水平显著升高，可能导致细胞结构损伤，并与多种疾病如心血管病、糖尿病、神经退行性疾病及癌症的发生发展密切相关。本试剂盒可为相关机制研究提供关键工具。

可选规格与价格

注：本产品仅供科研使用，不用于诊断、治疗或临床用途。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Reactive Oxygen Species (ROS) Assay Kit (Fluorometric Method)

Catalog Number: KTB1913-S

Product Type: Cell Metabolism Assay Kit

Application Fields: Research in cell metabolism, oxidative stress analysis, cardiovascular diseases, neurodegenerative diseases, cancer mechanism studies, etc.

Product Overview

This kit is a fluorometric-based tool for the detection of Reactive Oxygen Species (ROS), suitable for the quantitative analysis of ROS levels in live cells or fresh tissue samples. The kit utilizes the highly sensitive fluorescent probe DHE (Dihydroethidium), enabling efficient and reliable detection of ROS via flow cytometry or fluorescence microscopy.

Key Components

• DHE (1000×)
• ROS Positive Control (1000×)

Sample Types

Suitable for live cells and fresh tissue samples.

Detection Method

Fluorometric Assay, compatible with flow cytometry and fluorescence microscopy imaging analysis.

Features & Advantages

• High sensitivity, suitable for low-concentration ROS detection;
• Applicable to various sample types, offering high flexibility;
• Included positive control provides system quality control for more reliable results.

Precautions

• Pre-experiments are recommended to optimize detection conditions;
• Normalization of sample protein concentration using the BCA method is advised;
• Users must establish their own standard curve;
• Perform operations in a fume hood while wearing gloves and other protective equipment to avoid direct contact with reagents.

Storage & Shipping

Storage Conditions: Protect from light, store at -20°C, stable for 12 months.

Shipping Conditions: Shipped with ice packs (blue ice) to ensure reagent stability.

Warning

This product is a biochemical reagent with certain irritant and toxic properties. Strictly adhere to operating procedures.

Background Information

Reactive Oxygen Species (ROS) are byproducts of cellular aerobic metabolism, involved in cell signal transduction and homeostasis maintenance. Under oxidative stress, ROS levels significantly increase, potentially leading to cellular structural damage and are closely associated with the pathogenesis and progression of various diseases such as cardiovascular diseases, diabetes, neurodegenerative disorders, and cancer. This kit provides a key tool for related mechanism studies.

Available Specifications & Pricing

Note: This product is intended for research use only, not for diagnostic, therapeutic, or clinical purposes.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yaken Inc.