高铁还原酶试剂盒，用于样本中FCR活性检测，微量法，FCR

Micro Ferric Reductase (FCR) Activity Assay Kit | 高铁还原酶活性检测试剂盒

货号：KTB1621-S | 产品分类：核心产品 | 检测方法：比色法

产品概述

本试剂盒采用微量法设计，专为检测动植物组织、血清（浆）及其他液体样本中的高铁还原酶（FCR）活性而优化开发。基于酶促反应中Fe³⁺还原为Fe²⁺并与ferrozine显色的原理，可在562 nm波长下进行高特异性吸光度测定，适用于多种科研场景下的铁代谢机制研究。

检测原理

高铁还原酶（Ferric Reductase, FCR）在铁元素吸收途径中起关键催化作用，能够将高价铁螯合物中的Fe³�还原为Fe²⁺。生成的Fe²⁺与试剂中的ferrozine形成有色复合物，在562 nm处具有特征吸收峰，通过比色法可准确量化FCR酶活性。

组分清单（每盒）

• Extraction Buffer
• Reagent I
• Reagent II
• Standard

主要特点与优势

• 适用样本类型广泛，涵盖动植物组织、血清（浆）及多种液体样本
• 操作流程清晰简便，配套详尽样本制备与结果计算指南
• 提供标准品，有助于准确绘制标准曲线并提高数据可靠性

适用样本与检测范围

本品适用于多数常见动植物组织样本及血清（浆）、细胞裂解液等液体样本，可定量检测其中FCR酶活性水平。

保存与运输条件

保存条件：4℃避光保存，有效期6个月
运输方式：蓝冰冰袋运输，保障试剂稳定性

使用注意事项

• 建议使用新鲜样本进行检测。如需保存，请置于-80℃，避免反复冻融
• 样本解冻应在室温下快速完成（建议4小时内），以维持酶活性稳定
• 实验前请确保所有试剂、设备及环境温度符合要求

规格与定价

规格	价格（元）	货期
48 T/48 S	869	现货，次日发货
96 T/96 S	1439	现货，次日发货

警告：本产品仅限科研使用，不适用于人类或临床诊断。使用者应负责确保应用方式不侵犯任何专利或许可协议。

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Ferric Reductase (FCR) Activity Assay Kit

Catalog No.: KTB1621-S | Category: Core Products | Detection Method: Colorimetric Assay

Overview

This kit employs a microplate-based design, optimized for detecting ferric reductase (FCR) activity in animal and plant tissues, serum (plasma), and other liquid samples. Based on the enzymatic reduction of Fe³⁺ to Fe²⁺, which forms a colored complex with ferrozine, the assay enables high-specificity absorbance measurement at 562 nm, making it suitable for iron metabolism research in various scientific contexts.

Principle

Ferric reductase (FCR) plays a key catalytic role in iron uptake pathways by reducing Fe³⁺ in high-valence iron chelates to Fe²⁺. The generated Fe²⁺ forms a colored complex with ferrozine in the reagent, exhibiting a characteristic absorption peak at 562 nm, allowing accurate quantification of FCR enzyme activity via colorimetry.

Components (per kit)

• Extraction Buffer
• Reagent I
• Reagent II
• Standard

Key Features & Advantages

• Broad sample compatibility: applicable to animal/plant tissues, serum (plasma), and various liquid samples
• Clear and simple operating procedure with detailed sample preparation and calculation guidelines
• Includes standard for accurate curve establishment and improved data reliability

Applicable Samples & Detection Range

This product is suitable for most common animal/plant tissue samples, serum (plasma), cell lysates, and other liquid samples, enabling quantitative detection of FCR enzyme activity levels.

Storage & Shipping Conditions

Storage: Store protected from light at 4°C; stable for 6 months
Shipping: Shipped with blue ice packs to ensure reagent stability

Precautions

• Fresh samples are recommended. If storage is necessary, keep at -80°C and avoid repeated freeze-thaw cycles
• Thaw samples quickly at room temperature (recommended within 4 hours) to maintain enzyme activity stability
• Ensure all reagents, equipment, and ambient temperature meet requirements before experimentation

Specifications & Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	869	In stock, ships next day
96 T/96 S	1439	In stock, ships next day

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. User is responsible for ensuring that application does not infringe any patents or licensing agreements.

Note: The above information is subject to change due to product optimization and updates. Please consult Yakonin Inc. for detailed information.