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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃避光保存 6 个月
- 保质期:
-20℃避光保存 6 个月
- 英文名:
Luminescent ATP Detection Assay Kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
48 T/48 S/96 T/96 S
| 规格: | 48 T/48 S | 产品价格: | ¥598.0 |
|---|---|---|---|
| 规格: | 96 T/96 S | 产品价格: | ¥998.0 |
ATP试剂盒(发光法) - 细胞代谢检测核心解决方案
货号:KTB1019-S | 产品类型:细胞代谢检测试剂盒
产品概述
Luminescent ATP Detection Assay Kit是一款基于生物发光原理的高灵敏度ATP含量检测试剂盒。该产品设计用于精确测定动植物组织、血清(浆)及其他液体样本中的三磷酸腺苷(ATP)活性,是细胞代谢研究领域的核心工具。
检测原理
在镁离子和氧气存在条件下,萤火虫荧光素酶(Firefly Luciferase)催化底物(Luciferin)氧化生成氧化荧光素(Oxyluciferin),并释放光信号。光强度与ATP浓度在一定范围内呈线性正相关,从而实现定量分析。
试剂盒组分
- Extraction Buffer
- Assay Buffer
- Firefly Luciferase Substrate
- Luciferase
- ATP Standard (5mM)
特点与优势
- 验证样本类型齐全,涵盖多种生物样本
- 操作流程简便,适合高通量筛选
- 提供详尽的样本制备与计算结果方法指南
检测范围
适用于动植物组织、血清(浆)及其他液体样本中ATP活性测定。
注意事项
- 推荐使用新鲜样本,如需保存请置于-80℃(可存放数周)
- 解冻过程需严格控制温度与时间,室温解冻应在4小时内完成
- 实验前请确保所有组分及设备温度符合要求
保存与运输
保存条件:-20℃避光保存,有效期6个月
运输条件:冰袋(蓝冰)运输
规格与价格
| 规格 | 价格(元) | 货期 |
|---|---|---|
| 48 T/48 S | 598 | 现货(次日发货) |
| 96 T/96 S | 998 | 现货(次日发货) |
警告:本产品仅供研究使用,不适用于人类或临床诊断。使用前请确保符合相关专利及许可证要求。
别名:ATP,三磷酸腺苷 | 分子:ATP | 检测指标:ATP
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

ATP Assay Kit (Luminescent) - Core Solution for Cellular Metabolism Detection
Cat. No.: KTB1019-S | Product Type: Cellular Metabolism Assay Kit
Product Overview
The Luminescent ATP Detection Assay Kit is a highly sensitive kit based on the bioluminescence principle for detecting adenosine triphosphate (ATP) content. It is designed for the accurate determination of ATP activity in animal and plant tissues, serum (plasma), and other liquid samples, serving as a core tool in cellular metabolism research.
Assay Principle
In the presence of magnesium ions and oxygen, firefly luciferase catalyzes the oxidation of the substrate (luciferin) to form oxyluciferin, releasing a luminescent signal. The light intensity exhibits a linear positive correlation with ATP concentration within a certain range, enabling quantitative analysis.
Kit Components
- Extraction Buffer
- Assay Buffer
- Firefly Luciferase Substrate
- Luciferase
- ATP Standard (5mM)
Features & Advantages
- Comprehensive validated sample types, covering a variety of biological samples
- Simple operational procedure, suitable for high-throughput screening
- Provides detailed guidelines for sample preparation and calculation of results
Detection Range
Suitable for the determination of ATP activity in animal and plant tissues, serum (plasma), and other liquid samples.
Precautions
- Fresh samples are recommended. If storage is necessary, keep at -80°C (can be stored for several weeks)
- Strictly control temperature and time during thawing; thawing at room temperature should be completed within 4 hours
- Ensure all components and equipment meet temperature requirements before the experiment
Storage & Shipping
Storage Conditions: Store protected from light at -20°C; valid for 6 months
Shipping Conditions: Shipped with ice packs (blue ice)
Specifications & Pricing
| Specification | Price (¥) | Availability |
|---|---|---|
| 48 T/48 S | 598 | In stock (ships next day) |
| 96 T/96 S | 998 | In stock (ships next day) |
Warning: This product is for research use only. Not intended for human or clinical diagnostic use. Ensure compliance with relevant patents and licensing requirements prior to use.
Alias: ATP, Adenosine Triphosphate | Molecule: ATP | Detection Target: ATP
Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yacoin Company.
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
相关专题 在蛋白质定量试验中,最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒(BCA Protein Assay Kit)。因为BCA法蛋白定量具有很多优点,如1.实验操作十分简单方便;2.实验耗时短,快速;3.试剂灵敏度高,实验结果准确;4.所用的试剂稳定性好,而且经济实用,抗干扰能力强等。 实验原理: BCA(bicinchoninic acid)法蛋白浓度定量
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