FDH试剂盒，用于样本中甲醛脱氢酶活性检测，FDH Acti

FDH试剂盒 (Micro Formaldehyde Dehydrogen酶活性检测试剂盒) - 货号：KTB3033-E

产品类型：细胞代谢检测试剂盒
样本类型：适用于细胞样本
检测方法：比色法 (基于NADH在340 nm吸光值变化)

产品概述

本试剂盒专为检测样本中甲醛脱氢酶（FDH）活性而设计。FDH是一种广泛存在于原核和真核生物中的关键代谢酶，隶属于含锌中等链醇脱氢酶（ADH）家族。它通过催化甲醛与NAD+的反应生成NADH，在甲醛解毒代谢途径中发挥核心作用。

通过监测340 nm处吸光值的动态变化，可实现对FDH酶活性的精准、定量分析，为细胞代谢研究和毒性响应机制探索提供可靠工具。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ

保存与运输条件

保存建议：-20°C避光保存，有效期为6个月。
运输条件：冰袋运输（蓝冰），确保试剂稳定性。

注意事项

• 推荐使用新鲜样本进行检测。如不能立即实验，样本可在-80°C保存数周；解冻过程需严格控制温度与时间，室温解冻应在4小时内完成。
• 实验前请确保所有试剂及设备处于适宜温度，以保证检测结果的准确性。

产品规格与货期

背景说明

甲醛是一种高活性、高毒性化合物，可与蛋白质、核酸和脂类发生非特异性反应，对细胞功能造成严重影响。FDH作为甲醛氧化代谢中的关键酶，能够利用NAD+作为辅酶，催化甲醛氧化并生成NADH。通过监测340 nm处NADH吸光值的变化，可实现对FDH活性的灵敏、定量检测。

警告：本产品仅限研究使用，不适用于人类或临床诊断。使用前请确保符合相关专利与许可要求，本公司不对因使用本产品导致的专利侵权或其他违规行为承担责任。

别名：甲醛脱氢酶（FDH）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

FDH Assay Kit (Micro Formaldehyde Dehydrogenase Activity Assay Kit) - Cat. No.: KTB3033-E

Type: Cellular Metabolism Assay Kit
Sample Type: Suitable for cell samples
Assay Method: Colorimetric method (based on NADH absorbance change at 340 nm)

Overview

This kit is specifically designed to detect formaldehyde dehydrogenase (FDH) activity in samples. FDH is a key metabolic enzyme widely present in both prokaryotes and eukaryotes, belonging to the zinc-containing medium-chain alcohol dehydrogenase (ADH) family. It plays a central role in the formaldehyde detoxification metabolic pathway by catalyzing the reaction of formaldehyde with NAD+ to generate NADH.

By monitoring the dynamic changes in absorbance at 340 nm, precise and quantitative analysis of FDH enzymatic activity can be achieved, providing a reliable tool for research on cellular metabolism and exploration of toxic response mechanisms.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ

Storage and Shipping Conditions

Storage Recommendation: Store at -20°C protected from light; stable for 6 months.
Shipping Conditions: Shipped with ice packs (blue ice) to ensure reagent stability.

Precautions

• It is recommended to use fresh samples for the assay. If immediate testing is not possible, samples can be stored at -80°C for several weeks; the thawing process must be strictly controlled for temperature and time, and thawing at room temperature should be completed within 4 hours.
• Before the experiment, ensure all reagents and equipment are at appropriate temperatures to guarantee the accuracy of the assay results.

Specifications and Availability

Background

Formaldehyde is a highly reactive and toxic compound that can undergo non-specific reactions with proteins, nucleic acids, and lipids, severely impacting cellular functions. FDH, as a key enzyme in the oxidative metabolism of formaldehyde, utilizes NAD+ as a coenzyme to catalyze the oxidation of formaldehyde and generate NADH. By monitoring the change in NADH absorbance at 340 nm, sensitive and quantitative detection of FDH activity can be achieved.

Warning: This product is for research use only and is not intended for human or clinical diagnostic use. Ensure compliance with relevant patent and licensing requirements before use. The company is not liable for any patent infringement or other violations resulting from the use of this product.

Alias: Formaldehyde Dehydrogenase (FDH)

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.