Mitochondrial complex I试剂盒，用于样

线粒体呼吸链复合体Ⅰ活性检测试剂盒

货号：KTB1850-E

产品名称：Micro Mitochondrial complex I Activity Assay Kit（Mitochondrial complex I试剂盒）

产品概述

本试剂盒采用比色法定量检测多种样本类型（包括动物组织、植物组织及细胞样本）中线粒体呼吸链复合体Ⅰ（NADH-CoQ还原酶）的酶活性。该产品为细胞代谢与能量研究中的核心工具，适用于氧化磷酸化途径的相关机制研究。

检测原理

线粒体呼吸链复合体Ⅰ（EC 1.6.5.3）是线粒体内膜上的关键酶复合物，催化NADH的电子传递至辅酶Q，并在此过程中参与活性氧（ROS）的生成。本试剂盒通过酶促反应体系中吸光度的变化，精确反映复合体Ⅰ的催化活性，为研究电子传递链功能及氧化应激状态提供可靠依据。

组分列表

• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• ReagentⅣ
• ReagentⅤ
• ReagentⅥ

产品特点与优势

• 广泛适用于植物、动物组织及细胞样本的复合体Ⅰ活性检测；
• 提供详尽样本制备指南与结果计算方案，助力实验标准化与重复性；
• 无需特殊设备，常规分光光度计即可完成检测。

适用样本

动物组织、植物组织、细胞提取物等线粒体样本。

注意事项

• 请勿混用不同批次或不同厂家的试剂组分，以免影响结果准确性；
• 试剂复溶及移液过程中应避免产生气泡；
• 实验需使用新鲜制备的样本，并注意防止组分间交叉污染；
• 建议进行预实验以优化样本用量，若ΔA值偏高可使用ReagentⅡ适当稀释；
• 样本蛋白浓度需用户自行测定。

保存与运输

产品采用蓝冰冰袋运输，请在收到货后参照说明书保存各组分。在推荐保存条件下，自发货日起有效期为6个月。

规格与价格

警告：本产品仅用于科学研究，不适用于人类或临床诊断。使用前请查阅相关专利与许可协议，制造商对因使用本品可能造成的专利侵权或违规行为不承担责任。

别名：复合体1；线粒体呼吸链；线粒体呼吸链复合体；NADH脱氢酶检测试剂盒

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mitochondrial Complex I Activity Assay Kit

Catalog Number: KTB1850-E

Product Name: Micro Mitochondrial Complex I Activity Assay Kit (Mitochondrial Complex I Kit)

Product Overview

This kit utilizes a colorimetric method to quantitatively detect the enzymatic activity of mitochondrial respiratory chain Complex I (NADH-CoQ reductase) in various sample types, including animal tissues, plant tissues, and cellular samples. This product serves as a core tool in the study of cellular metabolism and energy, suitable for research on mechanisms related to the oxidative phosphorylation pathway.

Assay Principle

Mitochondrial respiratory chain Complex I (EC 1.6.5.3) is a key enzyme complex located in the inner mitochondrial membrane, catalyzing the transfer of electrons from NADH to coenzyme Q and participating in the generation of reactive oxygen species (ROS) during this process. This kit accurately reflects the catalytic activity of Complex I through changes in absorbance in the enzymatic reaction system, providing a reliable basis for studying electron transport chain function and oxidative stress status.

Component List

• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• ReagentⅣ
• ReagentⅤ
• ReagentⅥ

Features and Advantages

• Wide applicability for Complex I activity detection in plant, animal tissue, and cell samples;
• Provides detailed sample preparation guidelines and result calculation schemes to facilitate experimental standardization and reproducibility;
• No special equipment required; detection can be performed using a conventional spectrophotometer.

Applicable Samples

Mitochondrial samples such as animal tissues, plant tissues, and cellular extracts.

Precautions

• Do not mix reagents from different batches or manufacturers to avoid affecting result accuracy;
• Avoid generating bubbles during reagent reconstitution and pipetting;
• Use freshly prepared samples and prevent cross-contamination between components;
• Pre-experiments are recommended to optimize sample usage; if ΔA value is high, Reagent II can be used for appropriate dilution;
• Sample protein concentration must be determined by the user.

Storage and Shipping

The product is shipped with blue ice packs. Upon receipt, please store each component according to the instructions. Under recommended storage conditions, the product is valid for 6 months from the date of shipment.

Specifications and Pricing

Warning: This product is for research use only and is not intended for human or clinical diagnostic use. Please review relevant patents and licensing agreements before use. The manufacturer is not liable for any patent infringement or violations that may result from the use of this product.

Alias: Complex 1; Mitochondrial Respiratory Chain; Mitochondrial Respiratory Chain Complex; NADH Dehydrogenase Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakin Company.