GST试剂盒，用于样本中谷胱甘肽S-转移酶检测，GST As

GST试剂盒 (Micro Glutathione S-Transferase Assay Kit)

货号：KTB1630-E | 产品类型：细胞代谢检测试剂盒

产品概述

本GST试剂盒是一种基于比色法的检测工具，用于准确测定多种生物样本（包括血清、血浆、动植物组织/细胞裂解液及其他生物体液）中谷胱甘肽S-转移酶（GST）的活性。该试剂盒提供优化的反应组分与标准曲线，适用于研究细胞代谢、氧化应激及解毒机制等相关领域。

主要特点与优势

• 广泛样本适用性：可灵活应用于血清、血浆、动植物组织与细胞裂解液等多种样本类型。
• 详尽的操作指南：提供清晰的样本制备流程、反应条件优化建议及结果计算方法。
• 高精度标准曲线：经过系统优化，标准曲线具备良好的线性范围与重复性，有助于提高数据可靠性。
• 操作简便：试剂预配制，节省实验时间，降低操作复杂度。

检测原理

本试剂盒采用比色法，通过GST催化谷胱甘肽（GSH）与特定底物结合生成有色产物，在特定波长下测定吸光度值变化，从而定量GST酶活性。

试剂盒组分

• Assay Buffer
• Chromogen（显色剂）
• Substrate（底物）

适用样本与处理建议

适用于细胞裂解液、组织匀浆液、血清、血浆等样本。请注意：

• 样本处理需全程在冰上操作，以维持酶活性。
• 若需保存，样本可在-80℃条件下存放不超过一个月。
• 细胞样本提取时，建议使用Assay Buffer进行研磨或超声波处理，避免使用细胞裂解液。

实验注意事项

• 需使用96孔UV微孔板进行测定。
• 建议预先筛选2–3个差异较大的样本进行稀释预实验，确保反应在5分钟内呈线性。
• 若吸光度值超过1.0，建议对样本进行适当稀释并在最终计算时乘以稀释倍数。
• 反应温度需严格控制：25℃（一般物种）或37℃（哺乳动物）。
• 细胞样本中GST活性检测时，推荐细胞数量控制在3–5×10⁶之间。

保存与运输条件

• 保存条件：4℃避光保存。各组分具体存储条件请参见说明书。
• 有效期：自发货日起12个月。
• 运输方式：冰袋（蓝冰）运输。

规格与定价

规格	价格（元）	货期
48 T/48 S	278	现货，次日发货
96 T/96 S	426	现货，次日发货

背景知识

谷胱甘肽S-转移酶（GST）是一类广泛存在于细胞质中的多功能蛋白家族，是细胞内解毒系统的关键组成部分。它催化谷胱甘肽（GSH）与亲电子物质结合，增强其水溶性，促进其从胆汁或尿液排出，从而保护细胞免受化学物质损伤。此外，GST还具有非硒依赖性谷胱甘肽过氧化物酶（non-Se GSH-Px）活性，参与氧化损伤修复过程。

使用声明：本产品仅用于科学研究，不适用于人类或临床诊断。使用者应负责确保其应用不侵犯任何专利或许可协议。

别名：谷胱甘肽S-转移酶；GST检测；Glutathione S-Transferase Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

GST Assay Kit (Micro Glutathione S-Transferase Assay Kit)

Catalog Number: KTB1630-E | Product Type: Cell Metabolism Assay Kit

Product Overview

This GST Assay Kit is a colorimetric-based detection tool designed for the accurate determination of glutathione S-transferase (GST) activity in various biological samples, including serum, plasma, animal/plant tissue/cell lysates, and other biological fluids. The kit provides optimized reaction components and a standard curve, making it suitable for research in cell metabolism, oxidative stress, detoxification mechanisms, and related fields.

Key Features and Advantages

• Broad Sample Compatibility: Flexible application across multiple sample types such as serum, plasma, animal/plant tissues, and cell lysates.
• Comprehensive Protocol: Includes clear sample preparation procedures, reaction condition optimization suggestions, and result calculation methods.
• High-Precision Standard Curve: Systematically optimized to provide a broad linear range and high reproducibility, ensuring reliable data.
• User-Friendly Operation: Pre-formulated reagents reduce experimental time and operational complexity.

Assay Principle

This kit utilizes a colorimetric method. GST catalyzes the conjugation of glutathione (GSH) with a specific substrate to produce a colored product. The change in absorbance at a specific wavelength is measured to quantitatively determine GST enzyme activity.

Kit Components

• Assay Buffer
• Chromogen
• Substrate

Applicable Samples and Handling Recommendations

Suitable for cell lysates, tissue homogenates, serum, plasma, and other biological samples. Please note:

• Sample processing must be performed on ice to maintain enzyme activity.
• For storage, samples can be kept at -80°C for up to one month.
• For cell sample extraction, it is recommended to use Assay Buffer for grinding or ultrasonic treatment, avoiding the use of cell lysis buffer.

Experimental Considerations

• Use a 96-well UV microplate for measurement.
• Pre-screening 2–3 samples with significant differences for dilution pre-tests is advised to ensure the reaction remains linear within 5 minutes.
• If the absorbance value exceeds 1.0, dilute the sample appropriately and multiply by the dilution factor in final calculations.
• Strictly control reaction temperature: 25°C (general species) or 37°C (mammalian).
• For GST activity detection in cell samples, it is recommended to use 3–5×10⁶ cells.

Storage and Shipping Conditions

• Storage Conditions: Store at 4°C protected from light. Refer to the manual for specific storage conditions of each component.
• Shelf Life: 12 months from the date of shipment.
• Shipping Method: Shipped with ice packs (blue ice).

Specifications and Pricing

Specification	Price (¥)	Availability
48 T/48 S	278	In stock, ships next day
96 T/96 S	426	In stock, ships next day

Background

Glutathione S-transferase (GST) is a multifunctional protein family widely present in the cytoplasm and serves as a key component of the cellular detoxification system. It catalyzes the conjugation of glutathione (GSH) with electrophilic substances, enhancing their water solubility and promoting excretion via bile or urine, thereby protecting cells from chemical damage. Additionally, GST exhibits non-selenium-dependent glutathione peroxidase (non-Se GSH-Px) activity and participates in oxidative damage repair processes.

Usage Statement: This product is intended for research use only and is not suitable for human or clinical diagnostic applications. The user is responsible for ensuring that its application does not infringe upon any patents or licensing agreements.

Aliases: Glutathione S-Transferase; GST Assay; Glutathione S-Transferase Assay Kit

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult YaKeyin Company.