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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
收到货后,请避光保存于4℃。请参阅说明书中各个组件的存储条件,自发货之日起,按照推荐的保存条件,有效期为12个月。
- 保质期:
收到货后,请避光保存于4℃。请参阅说明书中各个组件的存储条件,自发货之日起,按照推荐的保存条件,有效期为12个月。
- 英文名:
Micro Reduced Glutathione (GSH) Assay Kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
48 T/48 S/96 T/96 S
| 规格: | 48 T/48 S | 产品价格: | ¥178.0 |
|---|---|---|---|
| 规格: | 96 T/96 S | 产品价格: | ¥298.0 |
Micro Reduced Glutathione (GSH) Assay Kit
产品概述
货号:KTB1600-E | 产品类型:细胞代谢检测试剂盒
本试剂盒是一款基于比色法原理,专业用于定量检测多种生物样本中还原型谷胱甘肽(GSH)含量的核心产品。其设计旨在为细胞代谢研究提供可靠、灵敏的分析工具,适用于包括血清、血浆、动植物组织、细胞裂解液及其他生物体液在内的广泛样本类型。
产品特点与优势
- 广泛的样本兼容性:可精确测定血清、血浆、动植物组织、细胞裂解物及其他生物体液中的还原型谷胱甘肽(GSH)含量。
- 优化的检测性能:提供了经过细致优化的标准曲线,确保检测结果的准确性与重复性。
- 高灵敏度与宽线性范围:检测灵敏度低至 2 µg/mL,线性检测范围覆盖 2 - 400 µg/mL,满足绝大多数实验需求。
- 完整的解决方案:试剂盒内含详细样品制备方案与结果计算方法,协助用户完成从样本处理到数据分析的完整流程。
试剂盒组分
- Extraction Buffer
- Assay Buffer
- Chromogen
- Standard
技术参数
- 检测方法:比色法
- 检测指标:还原型谷胱甘肽(GSH)
- 灵敏度:2 µg/mL
- 检测范围:2 - 400 µg/mL
规格与货期
| 规格 | 价格 (元) | 货期 |
|---|---|---|
| 48 T/48 S | 178 | 现货(次日发货) |
| 96 T/96 S | 298 | 现货(当天发货) |
保存与运输条件
- 保存建议:收到货后,请避光保存于4℃。请参阅说明书中各个组件的存储条件,自发货之日起,按照推荐的保存条件,有效期为12个月。
- 运输条件:冰袋运输(蓝冰)
注意事项
- 如不立即进行实验,样本可在-80°C条件下保存长达1个月。
- 正式测定前建议选择2-3个预期差异较大的样本进行预测定。
- 每次实验请使用新配制标准品,且配制后需在4小时内使用。
背景信息
还原型谷胱甘肽(GSH)是由甘氨酸、谷氨酸和半胱氨酸组成的内源性三肽,是细胞内最重要的抗氧化巯基化合物之一。它在维持血红蛋白还原状态、保护细胞免受氧化损伤、调节蛋白质巯基保护及氨基酸跨膜转运等方面发挥关键作用。还原型与氧化型谷胱甘肽的比值(GSH/GSSG)是反映细胞氧化还原状态的重要动态指标。通过检测GSH含量及GSH/GSSG比值,可有效评估细胞所处的氧化应激状态。
警告与用途
本产品仅供研究使用,不适用于人类或临床诊断。使用者应确保应用不违反任何专利或许可证规定。对于因使用本产品可能导致的专利侵权或其他违规行为,我们不承担任何责任。
别名与搜索关键词
谷胱甘肽 | 还原型谷胱甘肽(GSH)检测 | 还原型谷胱甘肽(GSH)检测试剂盒 | GSH | Reduced Glutathione | GSH检测
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Micro Reduced Glutathione (GSH) Assay Kit
Product Overview
Catalog Number: KTB1600-E | Product Type: Cell Metabolism Assay Kit
This kit is a core product designed for the quantitative detection of reduced glutathione (GSH) levels in various biological samples based on a colorimetric method. It provides a reliable and sensitive analytical tool for cell metabolism research and is suitable for a wide range of sample types, including serum, plasma, animal and plant tissues, cell lysates, and other biological fluids.
Features and Advantages
- Broad Sample Compatibility: Accurately measures reduced glutathione (GSH) levels in serum, plasma, animal and plant tissues, cell lysates, and other biological fluids.
- Optimized Detection Performance: Provides a meticulously optimized standard curve to ensure accuracy and reproducibility of results.
- High Sensitivity and Wide Linear Range: Detection sensitivity as low as 2 µg/mL, with a linear detection range of 2 - 400 µg/mL, meeting the needs of most experiments.
- Complete Solution: Includes detailed sample preparation protocols and result calculation methods to assist users through the entire workflow from sample processing to data analysis.
Kit Components
- Extraction Buffer
- Assay Buffer
- Chromogen
- Standard
Technical Parameters
- Assay Method: Colorimetric
- Target: Reduced Glutathione (GSH)
- Sensitivity: 2 µg/mL
- Detection Range: 2 - 400 µg/mL
Specifications and Availability
| Specification | Price (¥) | Availability |
|---|---|---|
| 48 T/48 S | 178 | In Stock (Ships Next Day) |
| 96 T/96 S | 298 | In Stock (Ships Same Day) |
Storage and Shipping Conditions
- Storage Recommendation: Upon receipt, store protected from light at 4°C. Refer to the manual for the storage conditions of individual components. The product is stable for 12 months from the date of shipment when stored under recommended conditions.
- Shipping Conditions: Shipped with ice packs (blue ice).
Notes
- If not used immediately, samples can be stored at -80°C for up to 1 month.
- It is recommended to perform a pilot assay with 2-3 samples showing expected significant differences before formal measurement.
- Always use freshly prepared standards for each experiment, and use them within 4 hours after preparation.
Background Information
Reduced glutathione (GSH) is an endogenous tripeptide composed of glycine, glutamate, and cysteine, and is one of the most important antioxidant thiol compounds in cells. It plays key roles in maintaining hemoglobin in a reduced state, protecting cells from oxidative damage, regulating protein thiol protection, and facilitating amino acid transmembrane transport. The ratio of reduced to oxidized glutathione (GSH/GSSG) is a crucial dynamic indicator of cellular redox status. Measuring GSH levels and the GSH/GSSG ratio can effectively assess the oxidative stress status of cells.
Warnings and Usage
This product is for research use only and is not intended for human or clinical diagnostic use. Users should ensure that their application does not infringe any patents or license regulations. We assume no liability for any patent infringement or other violations that may result from the use of this product.
Aliases and Search Keywords
Glutathione | Reduced Glutathione (GSH) Assay | Reduced Glutathione (GSH) Assay Kit | GSH | Reduced Glutathione | GSH Detection
Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult YaKeyin Corporation.
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
相关专题 在蛋白质定量试验中,最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒(BCA Protein Assay Kit)。因为BCA法蛋白定量具有很多优点,如1.实验操作十分简单方便;2.实验耗时短,快速;3.试剂灵敏度高,实验结果准确;4.所用的试剂稳定性好,而且经济实用,抗干扰能力强等。 实验原理: BCA(bicinchoninic acid)法蛋白浓度定量
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