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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃,避光保存 12 个月
- 保质期:
4℃,避光保存 12 个月
- 英文名:
Micro Direct Bilirubin (DBIL) Content Assay Kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
48 T/48 S/96 T/96 S
| 规格: | 48 T/48 S | 产品价格: | ¥226.0 |
|---|---|---|---|
| 规格: | 96 T/96 S | 产品价格: | ¥406.0 |
CheKine™ 微量直接胆红素(DBIL)含量检测试剂盒
货号:KTB1413-E | 产品分类:核心产品 | 产品中心:细胞分析
产品概述
CheKine™ 微量直接胆红素(DBIL)含量检测试剂盒是一款基于比色法原理开发的生化检测工具,专为精准定量血清或血浆样本中的直接胆红素(DBIL)含量而设计。该试剂盒通过测量450 nm波长下吸光度的变化,实现对DBIL的高特异性检测,检测范围宽广,灵敏度高,是进行细胞代谢研究和相关疾病模型研究的可靠工具。
检测原理
本试剂盒基于直接胆红素在特定氧化剂作用下被氧化生成胆绿素的生化反应。该反应会导致反应体系在450 nm波长处的吸光度下降,其下降程度与样本中直接胆红素的浓度成正比。通过建立标准曲线,即可精确定量计算出未知样本中的DBIL含量。
核心参数
- 检测方法:比色法
- 样本类型:血清、血浆
- 检测范围:1.0 - 400 μmol/L
- 灵敏度:1.0 μmol/L
- 规格选择:
- 48 T/48 S (现货,¥226)
- 96 T/96 S (现货,¥406)
试剂盒组分
- ReagentⅠ
- ReagentⅡ
- Standard
保存与运输
保存条件:4℃避光保存,有效期12个月。
运输条件:全程采用冰袋(蓝冰)低温运输,以确保试剂稳定性。
使用建议
为获得最佳检测结果,推荐使用新鲜采集的样本。若需保存,样本可在-80℃环境下冻存不超过1个月,应避免反复冻融。
应用背景
直接胆红素(DBIL)是胆红素代谢的重要指标,其血清水平异常升高与多种临床疾病密切相关,如各类肝炎、肝外胆道阻塞、溶血性疾病及新生儿黄疸等。本试剂盒为上述疾病的体外科学研究提供了一个稳定、高效的检测手段。
警告:本品仅供研究使用,不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定,对于因使用本产品而可能导致的专利侵权或其他违规行为,本公司不承担任何责任。
搜索关键词:直接胆红素, DBIL, 细胞代谢, 胆红素检测, 血清检测
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

CheKine™ Micro Direct Bilirubin (DBIL) Assay Kit
Cat. No.:KTB1413-E | Category:Core Products | Product Center:Cell Analysis
Overview
The CheKine™ Micro Direct Bilirubin (DBIL) Assay Kit is a biochemical tool developed based on the colorimetric principle, specifically designed for the accurate quantification of direct bilirubin (DBIL) levels in serum or plasma samples. By measuring absorbance changes at 450 nm wavelength, this kit enables highly specific detection of DBIL, featuring a broad detection range and high sensitivity. It serves as a reliable tool for research in cellular metabolism and related disease models.
Assay Principle
This kit is based on the biochemical reaction in which direct bilirubin is oxidized to biliverdin under the action of a specific oxidizing agent. This reaction results in a decrease in absorbance of the reaction system at 450 nm, and the extent of decrease is proportional to the concentration of direct bilirubin in the sample. By establishing a standard curve, the DBIL content in unknown samples can be precisely quantified.
Key Parameters
- Assay Method:Colorimetric method
- Sample Types:Serum, Plasma
- Detection Range:1.0 - 400 μmol/L
- Sensitivity:1.0 μmol/L
- Specifications:
- 48 T/48 S (In stock, ¥226)
- 96 T/96 S (In stock, ¥406)
Kit Components
- ReagentⅠ
- ReagentⅡ
- Standard
Storage and Shipping
Storage Conditions:Store protected from light at 4℃; stable for 12 months.
Shipping Conditions:Shipped with ice packs (blue ice) to maintain low temperature and ensure reagent stability.
Usage Suggestions
For optimal results, it is recommended to use freshly collected samples. If storage is necessary, samples can be stored at -80℃ for no more than one month, and repeated freeze-thaw cycles should be avoided.
Background
Direct bilirubin (DBIL) is a critical indicator of bilirubin metabolism. Abnormally elevated serum levels of DBIL are closely associated with various clinical diseases, such as different types of hepatitis, extrahepatic biliary obstruction, hemolytic diseases, and neonatal jaundice. This kit provides a stable and efficient detection method for in vitro research on these diseases.
Warning:This product is for research use only and is not intended for human or clinical diagnostic use. Users should ensure that their application does not infringe any patents or licensing regulations. The company assumes no liability for any patent infringement or other violations that may result from the use of this product.
Search Keywords:Direct Bilirubin, DBIL, Cellular Metabolism, Bilirubin Assay, Serum Assay
Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult YaKeYin Company.
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
相关专题 在蛋白质定量试验中,最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒(BCA Protein Assay Kit)。因为BCA法蛋白定量具有很多优点,如1.实验操作十分简单方便;2.实验耗时短,快速;3.试剂灵敏度高,实验结果准确;4.所用的试剂稳定性好,而且经济实用,抗干扰能力强等。 实验原理: BCA(bicinchoninic acid)法蛋白浓度定量
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
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