Β-amylase试剂盒，用于样本中β-淀粉酶活性检测，β-

Micro β-Amylase Activity Assay Kit | β-淀粉酶活性检测试剂盒

货号：KTB1380-E

产品概述

本试剂盒是一种基于比色法原理的高灵敏度检测工具，用于定量分析多种生物样本（包括血清、血浆、唾液、动植物组织等）中的β-淀粉酶活性。该产品属于细胞代谢检测领域的核心工具，适用于糖代谢相关研究。

检测原理

β-淀粉酶（β-Amylase, EC 3.2.1.2）能够催化淀粉分子中α-1,4糖苷键的水解，生成麦芽糖、葡萄糖等还原糖。本试剂盒通过DNS试剂与这些还原糖发生显色反应，在特定波长下测定吸光度值，从而准确计算酶活性。

组分列表

• DNS Reagent（显色剂）
• Substrate（底物）
• Standard（标准品）

特点与优势

• 可广泛适用于多种样本类型，包括动植物组织及体液样本
• 提供标准品及标准曲线，有助于准确定量与结果比对
• 配套详细的样本前处理与活性计算指南，操作明晰、重复性高
• 灵敏度高达0.0078 mg/mL，检测范围覆盖0.0156-1 mg/mL

规格与货期

保存与运输条件

4℃避光保存，有效期为12个月。本品采用蓝冰冰袋运输，以确保试剂稳定性。

注意事项

• 请勿混用不同批次或不同厂家的试剂组分
• 配制或复溶试剂时应避免产生气泡
• 实验过程中建议勤换吸头，防止交叉污染
• 操作前请确保所有试剂及设备已达适宜温度

应用声明

本产品仅限科学研究使用，不适用于临床诊断或人体应用。使用者应负责确保其应用不侵犯任何专利权或许可证规定，厂家不承担因产品使用而产生的专利侵权或其他违规责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro β-Amylase Activity Assay Kit

Catalog Number: KTB1380-E

Product Overview

This kit is a highly sensitive colorimetric assay designed for the quantitative determination of β-amylase activity in various biological samples, including serum, plasma, saliva, and plant or animal tissues. As a core tool in the field of cellular metabolism detection, it is suitable for research related to carbohydrate metabolism.

Assay Principle

β-Amylase (EC 3.2.1.2) catalyzes the hydrolysis of α-1,4 glycosidic bonds in starch molecules, producing reducing sugars such as maltose and glucose. This kit employs DNS reagent to react with these reducing sugars, generating a colorimetric product. The enzyme activity is accurately calculated by measuring the absorbance at a specific wavelength.

Component List

• DNS Reagent
• Substrate
• Standard

Features and Advantages

• Broad applicability to various sample types, including plant/animal tissues and body fluids
• Includes standard and standard curve for accurate quantification and result comparison
• Comes with detailed sample preparation and activity calculation guidelines, ensuring clear operation and high reproducibility
• High sensitivity (0.0078 mg/mL) with a detection range of 0.0156–1 mg/mL

Specifications and Availability

Storage and Shipping Conditions

Store protected from light at 4°C; stable for 12 months. Shipped with blue ice packs to ensure reagent stability.

Precautions

• Do not mix components from different batches or manufacturers
• Avoid bubble formation during reagent preparation or reconstitution
• Change pipette tips frequently during experiments to prevent cross-contamination
• Ensure all reagents and equipment have reached the appropriate temperature before use

Application Statement

This product is for research use only. Not intended for clinical diagnosis or human use. The user is responsible for ensuring that applications do not infringe upon any patents or licensing regulations. The manufacturer is not liable for patent infringement or other violations resulting from product use.

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Yakin Company.